Determination of berberine and trimethoprim in compound berberine in-jection by HPLC
Yin Xiaojian
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Bengbu Institute for Drug Control,Bengbu 233000
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History+
Received
Published
1994-06-15
1995-04-05
Issue Date
1995-04-05
Abstract
We used HPLC method for determination of berberine bisulfate and trimethoprimin compound berberine injection,The mobile phase consisted of a minxture of methanol ammoni-um acetate solution(1 mol/L,pH 5.5)(80:20).The flow rate was 1 ml/min。Wavelength was de-termined 265 nm。Phaenacetine was selected as internal standard. A ODS-C18 column was used。The standard curve of berberine bisulfate was linear in the range from 0. 10~0. 30 mg/ml. Theaverage recovery was 101.66%,RSD=1. 6%(n=6). The standard curve of trimethoprim was lin-ear in the range from 0. 25~1. 25 mg/ml.The average recovery was 101. 42%,RSD=1. 19%(n=6)。
Yin Xiojin.
Determination of berberine and trimethoprim in compound berberine in-jection by HPLC[J]. Chinese Pharmaceutical Journal, 1995, 30(04): 233-235