We proposed multiple linear regression spectrophotomerry for determining multi－ component mixture.The mathematical formula was ∑_mKiAi=kC。A set of standard liquid mix－ tures was prepared according to uniform design、and the absorption data of the mixture were measured at m wavelength．By means of computer，multilinear regression was used to process the absorption data of m wavelength and desired component concentrations of liquid mixtures in order to obtain working equation．The method was used to determine lidocaine hydrochloride of lido－ caine．Amethocaine injection and ephedrine hydrochloride of compound amethocaine injection．The mean recoveries were respectively 100．89％and 100．05％with RSD 0．56％and 0．34％．