In this experiment,PQS(100,300,500μg/ml)decreased the amplitude of actionpotential, overshooting, threshold, maximum diastolic potenlic,maximum rate of depolarizationand action potential duration at 50%level repolarization in cultivated rat cardiac cells. The effectsof PQS(300～500μg/ml) were similar to those of MnCl2(0.1μg/ml) and verapamil(1.0μmol/L)。The Ca￣(2+)(200mmol/L)could reverse the effect of PQS(300μg/ml)on inhibiting electricalparameter of action potential of rat cells. The results indicated that PQS may affect calcium channel block.
Key words
PQS /
myocardiocyte /
cell culture /
action potential
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
1任贵兴，王铁生，吕兴山，等.西洋参茎叶总皂甙、氨基酸及无机元素含量的研究.特产科学实验,1987，(4)：1.
2杨世杰,钟国赣,张文杰,等.西洋参塞叶皂甙对大鼠培养心肌细胞氧化损伤的保护作用.中国中药杂志，1992，9(17)：555.
3杨世杰,马兴元，矢岛道夫，等.西洋参サポニノの心筋取缩力に対すゐ而作用.愛知医科大学医学会志.1992,9(17）:555.
4李松云,郭力达,王健春，等?西洋参茎叶皂甙抗失血性休克及对心脏的保护作用.中华医学杂志，1990,7(70) ：411.
5赵光东,赵德化，盛宝恒.西洋参茎叶皂甙的抗实验性心律失常作用.第四军医大学学报，1987，8(5)：309.
6江岩，汪淑衍，张保国，等.硒与培养心肌细胞的自发性搏动.应用生理学杂志,1987,3(1)：26.
7Payct MD, Schannc OF, Ruiz-Ccrctti E. Compatition for slow channel of Ca2+,Mn2+,verapamil, and D-600 in rat ventricular muscle. Moi Cell Cardiol, 1980,12 ： 635.
8Sperelakis N. Electrophysiology of calcium antagonist. J Mol cell cardiol，1987,19(Suppl Ⅱ) : 19.
9Tsien RM, Hess P, McCIeskey EW, et al. Calcium chan nels ： Mechanisms of electivity permeation and blocd.Ann Rev Biophys Chem，1987,16 : 265.
10Lee KS, Tsien RW. Mechanism of calcium channel blockade by verapamil, D 600； diltiazem and nitrendipine in single dialyed heart cells. Nature,19S3;302 : 790a.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
