OBJECTIVE To seek for some traditional Chinese medicine that can reverse bacterial drug resistance, in order to provide a new solution to the threat of super-drug resistance. METHODS Blackberrylily, Coptis, Radix Scutellariae, Rhubarb, and the compound prescription of Radix Scutellariae and Rhubarb(named double-yellow decoction 1), Coptis and Rhubarb(named double-yellow decoction 2), Radix Scutellariae, Rhubarb and Coptis (named three-yellow decoction),which is consisting of Coptis, Radix Scutellariae and Rhubarb,were selected, then the extracts were decocted. The bacterial resistance reversal test was developed on the basis of the MIC the extracts to Acinetobacter baumannii, subMIC as the work concentration. The MIC of ciprofloxacin was detected before and after cultured in medium containing extracts to evaluate whether the drug resistance reversed in bacteria. Then the bacteria plasmids were extracted and the electrophoresis map analyzed to identify whether some plasmids lost. RESULTS The reverse rate of Blackberrylily, Coptis, Radix Scutellariae, Rhubarb,double-yellow decoction 1,double-yellow decoction 2,three-yellow decoction was 4.2%, 2.8%, 6.9%, 6.9%,11.1%, 2.8% and 11.1%, respectively. The effect of double-yellow decoction 2 is more obvious. The relevance of plasmid was 33.3%, 100%, 0%, 20%, 75%, 50% and 25%,the effect of Coptis is more obvious. CONCLUSION The extract of Coptis and Rhubarb (double-yellow 1) is best for reversing bacterial drug resistance.
Key words
traditional Chinese medicine /
reverse of resistance /
multi-resistant bacteria /
Blackberrylily /
Coptis /
Radix Scutellariae /
Rhubarb /
screening
{{custom_keyword}} /
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] YU H, LI Y L. Traditional Chinese medicine reverse the research progress of drug resistance of bacteria[J]. China Anim Husb Vet Med(中国畜牧兽医), 2005, 32(1): 54-56.[2] LI C Q, ZHOU Q X, LING B D. Status and prospects of resistance plasmid elimination[J]. J North Sichuan Med Coll(川北医学院学报),2003, 18(2): 168-171.[3] National Committee for clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests. Approved standard[S]. NCCLS(美国临床实验室标准化协会),2006,M2-A9,9th ed.[4] WEN X M, REN N, WU A H, et al. Antimicrobial resistance of bacteria and changing trend in China nosocomial infection surveillance system[J]. Chin J Infect Control(中国感染控制杂志), 2009, 8(6):389-396.[5] ALICIA I H, JONATHAN R E, JEAN P. Antimicrobial-resistant pathogens associated with healthcare-associated infections: Annual summary of data reported to the national healthcare safety network at the centers for disease control and prevention, 2006-2007[J]. Infect Cont Hosp Epidemiol, 2008, 29(11):996-1011.[6] KARTHIKEYAN K K, MARK A T,TIMOTHY R W. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: A molecular, biological,and epidemiological study[J]. Lancet Infect Dis, 2010, 10(9): 597-602.[7] LIVERMORE D M. Fourteen years in resistance[J]. Int J Antimicrob Agents, 2012, 39(4): 283-294.[8] ZHANG Y B, ZHANG K X, TANG Y C, et al. Study on β-lactams resistance and genotype of β-lactamases in plasmid-mediated AmpC β-lactamases and ESBLs producing bacteria[J]. Chin J Microbiol Immunol(中华微生物学和免疫学杂志),2004,24(7): 577-582.[9] WANG Q, CHEN Y, CHENG J, et al. Resistance and associated resistance mechanism of Escherichia coli producing plasmid-mediated AmpC β-lactamases[J]. Chin J Antibiot(中国抗生素杂志), 2008, 33(2): 106-110.[10] HANG Y F, XUE X Y, FANG Y, et al. Progress of traditional Chinese medicine antibacterial and reversal of drug resistance mechanism[J].J China Pharm(中国药房), 2011, 22(44): 4504-4507.
{{custom_fnGroup.title_en}}
Footnotes
{{custom_fn.content}}
PDF(941 KB)
