目的建立山楂中枸橼酸的高效液相含量测定方法。方法 高效液相色谱法,Zorbax-sb C₁₈柱,磷酸二氢铵-磷酸缓冲液为流动相,流速1.0 mL·min^-1;检测波长为210nm;柱温25℃。结果枸橼酸回归方程Y=3510.8+56992.3X,r=0.999 9,线性范围0.79～15.96μg。生山楂中枸橼酸平均回收率为103.4％,RSD=0.9％;焦山楂中枸橼酸平均回收率为98.8％,RSD=2.8％(n=5)。结论该方法简便易行,可用于山楂饮片中枸橼酸的含量测定。

OBJECTIVE To establish a RP-HPLC method for the determination of citric acid in Fructus Crataegi and Fructus Crataegi Preparatus, and to analyse the content of citric acid in samples collected from different regions. METHODS A Zorbax sbC₁₈ (250 mm×4.6 mm,5μm) column was used with phosphate buffer solution (pH 3.0) as the mobile phase at a flow rate of 1.0 mL·min^-1.The column temperature was 251 and the UV detection wavelength was 210nm. RESULTS The calibration curve for citric acid was in good linearity over the range of 0.79～15.96μg with the regression equation Y=3510.8 + 56 992.3X,r=0.999 9.The average recoveries of citric acid in Fructus Crataegi and Fructus Crataegi Preparatus were 103.4% and 98.8%,respectively.CONCLUSION The method is simple, sensitive and reproducible .