摘要
目的 运用反相高效液相色谱法（RP-HPLC）同时测定石胆草药材中一个苯乙醇苷成分(PGs)和3个黄酮碳苷成分(FC-GsⅠ、FC-GsⅡ、FC-GsⅢ)的含量。方法 研究优化了石胆草药材中主要成分的检测波长和色谱分离条件等，采用ZORBAX SB-C18(4.6 mm×250 mm，5 μm)色谱柱，以乙腈-四氢呋喃（10∶1）-0.02%磷酸为流动相，梯度洗脱，检测波长为332 nm，石胆草中4个主要成分得到分离（Rs>1.5），历时45 min。结果 4种成分线性关系良好，r均大于0.999 0，线性范围分别为0.4~3.2 μg (PGs)、0.32~2.56 μg (FC-GsⅠ)、0.4~3.2 μg (FC-GsⅡ)、0.46~3.52 μg (FC-GsⅢ)，方法平均加样回收率>98.9%，相对标准偏差RSD<2.08%(n=6)。结论 此方法简便、快速、准确，适合用于石胆草药材的质量控制。
Abstract
OBJECTIVE To develop a reversed-phase high-performance liquid chromatography (RP-HPLC) procedure for simultaneous determination of one phenylethanoid glycosides (PGs) and three flavonoid C-glycosides (FC-GsⅠ, FC-GsⅡ and FC-GsⅢ) in Boea hygrometrica (Bunge) R. Brown. METHODS The detection wavelength and chromatographic separation condition were optimized. ZORBAX SB-C18 column (4.6 mm×250 mm，5 μm) was used. The 4 main components were separated with acetonitrile： tetrahydrofuran (10∶1) - 0.02% phosphate in 45 min with good resolution (Rs>1.5). The components were detected at 332 nm. RESULTS All correlation coefficients were greater than 0.999 0, and the linear ranges were 0.4-3.2 μg (PGs), 0.32-2.56 μg (FC-GsⅠ), 0.4-3.2 μg (FC-GsⅡ) and 0.46-3.52μg (FC-GsⅢ), respectively. The average recoveries were greater than 98.9%, and the relative standard deviations (RSD) were less than 2.08% (n=6). CONCLUSION The method is rapid, convenient and accurate, which can be used as the quality control method for Boea hygrometrica (Bunge) R. Brown.
关键词
反相高效液相色谱法 /
石胆草 /
苯乙醇苷 /
黄酮碳苷 /
含量测定
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Key words
RP-HPLC /
Boea hygrometrica (Bunge) R. Brown /
phenylethanoid glycosides /
flavonoid C-glycosides
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王彦志 刘媛媛 郑晓珂 贾玉光 冯志毅.
RP-HPLC同时测定石胆草中4种主要成分的含量[J]. 中国药学杂志, 2011, 46(19): 1517-1519
WNG Yn-zhi;LIU Yun-yun;ZHENG Xio-ke;JI Yu-gung;FENG Zhi-yi.
A Reversed-phase High Performance Liquid Chromatography (RP-HPLC) Procedure for Determination of Four Main Components in Boea hygrometrica (Bunge) R. Brown[J]. Chinese Pharmaceutical Journal, 2011, 46(19): 1517-1519
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参考文献
[1] ZHENG X K， LI J. Advances in study of Gesneriaceaes [J]. Chin New Drugs J (中国新药杂志)， 2003， 12（4）： 261-263.
[2] ZHENG X K， LI J， FENG W S， et al. Studies on phenylethanoid glycosides from Corallodiscus flabellata [J]. Chin Tradit Herb Drugs(中草药)， 2002， 33（10）： 881-883.
[3] ZHENG X K， LIU Y B， LI J， et al. One new phenylethanoid glycoside from Corallodiscus flabellata[J]. Acta Pharm Sin (药学学报)， 2002， 39（9）： 716-718.
[4] FENG W S， ZHENG X K， LIU Y B， et al. Isolation and structural identification of C-glycosylflavones from Corallodiscus flabellata[J]. Acta Pharm Sin (药学学报)， 2004， 39（2）： 110-115.
[5] JING H， ZUO J F， LI J S. Pharmacological research development of phenylethanoid glycosides [J]. Lishizhen Med Mater Med Res (时珍国医国药)， 2006， 17（3）： 440-441.
[6] WU X A， ZHAO Y M. Development of natural c-flavonoids glucoside and its active research [J]. Pharm J PLA (解放军药学学报)， 2005， 21(2)： 135-138.
[7] GAO H M， WANG Z M， QUN L， et al. Determination of calceolarioside B in Caulis Akebiae by RP-HPLC[J]. China J Chin Mater Med (中国中药杂志)， 2007， 32(6)：476-478.
[8] ZHANG F， SUN L N， CHUN W S.Content determination and quality evaluation of phenylethanoid glycosides in different origin Lamiophlomis rotata(Benth.)Kudo[J]. China J Chin Mater Med (中国中药杂志)， 2008， 33(11)：1346-1347.
[9] QU C H， YAN J， TIAN J M， et al. Determination of three kinds of flavonoid glycosides in Trollius chinensis Bunge. by HPLC [J]. Chin Tradit Pat Med(中成药)， 2010， 32(3)：500-502.
[10] LI H Y， DAI S W， SHOU D， et al. Study of puerarin in redix puerariae by reversed-phase high performance liquid chromatography[J]. China Pract Med (中国实用医药)， 2008， 3(7)：7-8.
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