摘要
目的研究翠云草中的双黄酮类成分。方法利用Sephadex LH-20、硅胶等色谱技术进行分离纯化,用UV,IR,NMR,MS等方法确定结构。结果分离得到5个双黄酮化合物,分别鉴定为穗花杉双黄酮(Amentoflavone,Ⅰ)、罗波斯塔双黄酮(Robustaflav-dimethyl ether,Ⅲ)、2″,3″-二氢-4′-甲氧基穗花杉双黄酮(2″,3″-dihydro-4′-O-methyl Amentoflavone,Ⅳ)、7″-甲氧基-2,3,2″,3″-四氢扁柏双黄酮(7″-O-methyl-2,3,2″,3″-tetrahydrohinokiflavone,Ⅴ)。结论化合物Ⅱ,Ⅲ为首次从该植物中分离得到,化合物Ⅳ,Ⅴ为新化合物。
Abstract
OBJECTIVE To study the biflavonoids of Selaginella uncinata(Desv.) Spring.METHODS The biflavonoids were isolated and purified by column chromatography on silica gel column and Sephadex LH-20 column.Their structure were determined by spectroscopic methods including UV,IR,NMR and MS.RESULTS Five biflavonoids including amentoflavone,robustaflavone,robustaflavone-4′-dimethyl ether,2″,3″-dihydro-4′-O-methylamentoflavone,7″-O-methyl-2,3,2,3′-tetrahydrohinokiflavone were isolated from S.uncinata(Desv.) Spring.CONCLUSION Compound Ⅱ,Ⅲ are firstly found in this plant.Compound Ⅳ,Ⅴ are new compounds.
关键词
翠云草 /
罗波斯塔双黄酮 /
罗波斯塔-4′-甲醚 /
2″ /
3″-二氢-4′-甲氧基穗花杉双黄酮 /
7″-甲氧基-2 /
3 /
2″ /
3″-四氢扁柏双黄酮
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Key words
S.uncinata /
robustaflavone /
robustaflavone-4′-dimethyl ether /
2″ /
3″-dihydro-4′-O-methylamentoflavone /
7″-O-methyl-2 /
3 /
2″ /
3″-tetrahydrohinokiflavone
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范晓磊;徐嘉成;林幸华;陈科力.
翠云草中双黄酮类成分研究[J]. 中国药学杂志, 2009, 44(01): 15-19
FN Xio-lei;XU Ji-cheng;LIN Xing-hu;CHEN Ke-li.
Study on Biflavonoids from Selaginella uncinata(Desv.) Spring [J]. Chinese Pharmaceutical Journal, 2009, 44(01): 15-19
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参考文献
[1] LUO J F. Study and summary on fern which can be use to anticancer in Miao nationality[J] . Chin J Ethnomed Ethnopharm (中国民族医药杂志)，1994，（9）36-37.
[2] LEE H S，WON K O，BO Y K，et al. Inhibition of phospholipase CY1 activity by amentoflavone isolated from Selaginella tamariscina [J] . Planta Med，1996，62(4)：293-296.
[3] MA L Y，MA S C，WEI F，et al. Uncinoside A and B，two new antiviral chromone glycosides from Selaginella uncinata [J]. Chem Pharm Bull (Tokyo)，2003，51(11)：1264-1267.
[4] LI L，CHEN K L，ZHU T M，et al. Investigation on xantine oxidase inhibitory activities in vitro of extracts from the medicinal plants of Selaginella genus[J] . J Chin Med Mater (中药材)，2007，30(4)：445-447.
[5] LI L，CHEN K L，ZHU T M，et al. Inhibition of lipoxygenase by the extracts from the medicinal plants of Selaginella genus[J] . Chin J Hosp Pharm (中国医院药学杂志)，2006，26（12）：1514-1517.
[6] LEE S，CHEN W A，ZENG T，et al. Biflavones of Selagnella doederlenii Hiercn[J]. Chin Pharm J (中国药学杂志)，1992，44(6)：537.
[7] KENNETH R，CAROLYN S，HANS G. 13C-NMR studies of some naturally occurring amemtoflavone and hinokiflavone biflavonoids [J] . Phytochemistry，1987，20(12)：3335-3337.
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脚注
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基金
国家自然科学基金资助项目(30470193)
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