摘要
目的建立顶空毛细管气相色谱法测定猪肺表面活性物质中的有机溶剂残留量。方法采用HP-624毛细管柱,顶空进样法,FID检测器,以正丙醇为内标进行测定。结果甲醇、丙酮、氯仿的线性范围为17.55～877.3 mg·L-1(r=0．999 9), 29.62～1 481.8 mg·L-1(r=1.000 0),0.358 2～17.91 mg·L-1(r=0.999 8);平均回收率分别为102.7％,98.1％,99.3％;RSD分别为1.5％,1.3％,2.9％(n=9)。结论本方法简单、准确、灵敏度高、重现性好,适用于猪肺表面活性物质中有机溶剂残留量的测定。
Abstract
OBJECTIVE To develop a headspace capillary gas chromatography method for the determination of residual organic solvents in porcine pulmonary surfactant.METHODS The samples were injected into HP-624 capillary column by headspace sampling and analyzed by using n-propanol as internal standard with FID detector.RESULTS The linear ranges of methanol, acetone and chloroform were 17.55～877.3 mg·L-1(r=0.999 9) ,29.62～1 481.8 mg·L-1(r=1.000 0)and 0.358 2-17.91 mg·L-1(r=0.999 8),respectively.The recoveries were 102.7%,98.1% and 99.3% with RSDs of 1.5% ,1.3% and 2.9% , respectively (n=9). CONCLUSION The method is simple, accurate and sensitive with good reproducibility.It can be used for the determination of the residual organic solvents in porcine pulmonary surfactant.
关键词
猪肺表面活性物质 /
顶空毛细管气相色谱法 /
有机溶剂残留
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Key words
porcine pulmonary surfactant /
headspace capillary gas chromatography /
residual organic solvents
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叶小敏;李慧义;张启明;冯芳;田颂九.
顶空毛细管气相色谱法测定猪肺表面活性物质的有机溶剂残留量[J]. 中国药学杂志, 2006, 41(01): 60-62
YE Xio-min;LI Hui-yi;ZHNG Qi-ming;FENG Fng;TIN Song-jiu.
Determination of Residual Organic Solvents in Porcine Pulmonary Surfactant by Capillary Gas Chromatography with Headspace Sampling [J]. Chinese Pharmaceutical Journal, 2006, 41(01): 60-62
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参考文献
[1] CHEN C. The exploitation and clinical application of pulmonary surfactant[J] . World Clin Drugs(世界临床药物)，2003，24(7)：407-412.
[2] ChP(2000)Vol Ⅱ(中国药典2000年版.二部)[S] .2000：Appendix 64，161.
[3] ZHOU H J. Technical Requirements for Registration of Pharmaceuticals for Human Use Quality(药品注册的国际技术要求质量部分)[M] . Beijing： People's Medical Publishing House，2000：79-100.
[4] FOCH F，LESS M，STANLEY G H S. A simple method for the isolation and purification of total lipids from animals tissues[J] . J Biol Chem，1957，226(1)：497-509.
[5] ZHAO R S， HOU X P， YAN B X. Preparation and studies on the quality standard of the liposomes carrier materials lecithin[J] . Chin J Mod Appl Pharm(中国现代应用药学杂志)，2001，18(1)：39-40.
[6] YU J，QIU A Y，LIU Y F. Advances in research of phosphatide[J] . China Western Cereals & Oils Technology（西部粮油科技），2000，25(1)：26-29.
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脚注
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