目的 研究姜黄的超临界CO2萃取工艺及其解酒护肝作用。方法 以萃取物得率、姜黄素和α-姜黄烯含量为评价指标,采用单因素试验和正交试验设计对夹带剂加入量、萃取压力、萃取温度、萃取时间、分离釜压力和温度等工艺参数进行优化,采用小鼠醉酒模型、大鼠醉酒模型和小鼠亚急性酒精性肝损伤模型评价姜黄超临界萃取物的解酒功效、途径和护肝作用。结果 姜黄超临界CO2萃取的最佳工艺为:萃取压力30 MPa,萃取温度55 ℃,加入药材重量1 倍的乙醇作为夹带剂,萃取时间2 h,分离釜压力与储罐压力一致,分离釜温度40 ℃,50 ℃减压回收乙醇。此外,姜黄超临界萃取物可以显著降低小鼠醉酒后的睡眠时间和醒酒实验(P<0.01),提高大鼠体内的乙醇脱氢酶(alcohol dehydrogenase, ADH)平(P<0.01),还可以降低亚急性肝损伤模型小鼠的血清中总胆固醇(total cholesterol, TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol, LDL-C)和总胆红素(total bilirubin, TBIL)水平(P<0.01)。结论 本研究制备工艺萃取率高、稳定可行、制备的姜黄超临界萃取物具有较好的解酒功效和降低亚急性酒精性肝损伤作用。
Abstract
OBJECTIVE To explore the supercritical CO2 extraction process of Curcuma longa L. and its effects on alleviating alcohol intoxication and protecting the liver. METHODS The extraction yield, curcumin, and α-turmerone contents were used as evaluation indicator. Single-factor experiments and orthogonal experimental designs were employed to optimize process parameters such as the amount of co-solvent, extraction pressure, extraction temperature, extraction time, separation vessel pressure, and temperature. The alcohol alleviating effects, pathways, and liver protection of Curcuma longa L. Supercritical CO2 extract were evaluated using mouse alcohol intoxication models, rat alcohol intoxication models, and mouse subacute alcohol-induced liver injury models. The results indicated that the optimal process for Curcuma longa L. RESULTS Supercritical CO2 extraction included: an extraction pressure of 30 MPa, extraction temperature of 55 ℃, using ethanol as a co-solvent at a weight equal to that of the medicinal material, an extraction time of 2 hours, maintaining consistent pressure in the separation vessel and storage tank, a separation vessel temperature of 40 ℃, and ethanol recovery through depressurization at 50 ℃. Furthermore, the supercritical extract significantly reduced the sleep time of mice after alcohol intoxication and improved their wake-up experiment (P<0.01), increased the ADH level in rats (P<0.01), and reduced the levels of TC, LDL-C, and TBIL in the serum of mice in the subacute liver injury model (P<0.01). CONCLUSION The preparation process developed in this study has a high extraction rate, is stable and feasible, and the supercritical CO2 extract produced exhibited good alcohol alleviating effects and reduces subacute alcohol-induced liver injury.
关键词
姜黄 /
超临界CO2萃取 /
工艺优化 /
解酒功效 /
肝损伤
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Key words
Curcuma longa L. /
supercritical CO2 extraction /
process optimization /
alcohol-alleviating effect /
liver damage
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参考文献
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脚注
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基金
云南特色植物筛选与研发服务CXO平台建设项目资助(2022YKZY001)
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