1. School of Chemistry and Molecular Engineering，East China University of Science and Technology，Shanghai 200237，China; 2. Department of Chemistry，Tsinghua University，Beijing 100084，China
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History+
Received
Revised
Published
2008-03-02
2008-03-02
2008-03-02
Issue Date
2009-01-10
Abstract
OBJECTIVE To characterize the chemical structure of a homogenous polysaccharide SDC obtained from Mulberry leaves. METHODS Sugar component analysis,methylation analysis,partial acid hydrolysis,IR,1H-NMR,13C-NMR were applied to determine the structural features. RESULTS The molecular weight of SDC was 8.0×103 dalton by HPGFC. SDC consisted of rhammose (Rha),arabinose (Ara),xylose (Xyl),galactose (Gal) and galacturonic acid units (GalA) in the molar ratio of 1.0∶1.6∶ 1.2∶2.0∶41.7. Part of galacturonic acid existed of methyl ester. SDC possessed an α-(1→4)-galacturonan backbone. The neutral side chains were attached to the O-3 ,O-2 of GalA residues including terminal,1,5-linked,1,3,5-linked Ara,terminal Xyl and terminal,1,6-linked Gal. CONCLUSION SDC was a new pectic polysaccharide for the first time obtained from Muberry leaves.
XI Wei;LIU Shu-qi;SHEN Fng-hong;ZHNG Wen-qing;LUO Guo-n.
Studies on Structural Features of Polysaccharide from Mulberry leaves [J]. Chinese Pharmaceutical Journal, 2009, 44(02): 99-102
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References
[1] CHENG F Y，YE S Y. Studies on mulberry leaves on anti-diabetes[J]. J Pharm Pract(药学实践杂志) ，2005，23(2)：71-74.
[2] SUN L，MENG L，YAN C，et al. Active components of lowering blood glucose and their pharmacological effect of mulberry leaves[J].Chin Tradit Herb Drugs(中草药)，2002，33(5)： 471-473.
[3] YUAN A H，MA J，JIANG X F，et al. Screening of glucosidase inhibitors from various fractions of mulberry leaves[J].Chin J Chin Mater Med (中国中药杂志)，2006，31(3)：223-227.
[4] CHEN F J，LU J，ZHANG Y Y. Pharmacological studies on Morus (I)：Effect of total polysaccharides of Morus(TPM)on carbohydrate metabolism in diabetic mice[J].J Shenyang Pharm Univ (沈阳药科大学学报)，1996，13(1)：24-27.
[5] CHEN Y，XUE Z. Studies on the polysaccharide of Morus alba L[J]. J China Japan Friendship Hosp (中日友好医院学报)，1990，4(4)：210-212.
[6] OU Y Z，CHEN J，LI Y H. Separation，purification and composition analysis of polysaccharides in leaves of Morus alba[J]. Food Sci (食品科学)，2005，26(3)：181-184.
[7] JIN C Y，WU T ，SHEN Q，et al. Studies on separation，purification of acid proteoglycanfrom folium mori(APFM) and its strucutures[J]. Food Sci (食品科学)，2007，28(6)：43-47.
[8] ZHANG W J. Biochemical Study Technology of Complex Polysaccharides(复合多糖生化研究技术) [M].2nd ed，Hangzhou：Zhejiang University Press，1999：40-41.
[9] TAYLOR R L，CONRAD H E.Stoichiometric depolymerization of polyuronides and glycosaminoglyc- uronans to monosaccharides following reduction of their carbodiimide-activated carboxyl groups[J]. Biochem，1972，11(8)：1385-1388.
[10] NEEDS P W，SEVENDRAN R R. Avoiding oxidative degradation during sodium hydroxide methyl iodide-mediated carbohydrate methylation in dimethyl sulfoxide[J]. Carbohydr Res，1993，245(1)：1-10.
