摘要
目的研究3种前体化合物和几种非生物诱导子对丹参(Salvia miltiorrhizaBge)不定根生长及其有效成分含量丹参酮ⅡA和原儿茶醛的影响。方法通过添加前体化合物和诱导子两阶段培养丹参不定根,探讨其对丹参不定根生长和丹参酮ⅡA、原儿茶醛含量的影响。结果培养第0天饲喂前体化合物,发现添加1.0 mmol·L-1乙酸钠、0.5 mmol·L-1酪氨酸和0.5 mmol·L-1乙酸钠分别得到了最高的丹参不定根增殖倍数、丹参酮ⅡA含量和原儿茶醛含量。培养第16天各种非生物诱导子处理,发现超声处理丹参不定根产量和原儿茶醛含量明显增加;1.0 mg·L-1水杨酸促进丹参酮ⅡA合成;高盐和高糖促进丹参不定根生长和次生代谢物合成;硝酸银和硝酸镧促进丹参酮ⅡA合成,但抑制了原儿茶醛合成。结论前体物和诱导子两阶段培养对于丹参酮ⅡA和原儿茶醛合成有显著影响。
Abstract
OBJECTIVE To investigate the effects of precursors and elicitors feedings on the growth of Salvia miltiorrhiza adventitious roots and the contents of tanshinone ⅡA and protocatechuic aldehyde.METHODS The adventitious roots were obtained through tissue culture by feeding precursors and elicitors,and the contents of tanshinone ⅡA and protocatechuic aldehyde were determined by HPLC.RESULTS The highest root growth rate and the content of tanshinone ⅡA and protocatechuic aldehyde were acquired by feeding precursors of 1.0 mmol·L-1 sodium acetate,0.5 mmol· L-1 tyrosine and 0.5 mmol·L-1 sodium acetate on day 0 during cultivation.Some kinds of abiotic elicitors were fed on day 16 during cultivation.The results showed that ultrasonic stimulation advanced root growth and protocatechuic aldehyde accumulation,1.0 mg·L-1 salicylic acid treatment was favorable for tanshinone ⅡA synthesis,the addition of high sugar and high salt greatly improved root growth and secondary metabolite yields,and the addition of silver nitrate and lanthanum nitrate advanced tanshinone ⅡA formation while inhibited protocatechuic aldehyde formation.CONCLUSION Tissue culture by feeding precursors and elicitors had distinct effects on the biosynthesis of tanshinone ⅡA and protocatechuic aldehyde.
关键词
丹参 /
前体物 /
诱导子 /
丹参酮ⅡA /
原儿茶醛
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Key words
Salvia miltiorrhiza /
precursors /
elicitors /
tanshinone ⅡA /
protocatechuic aldehyde
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郭肖红;高文远;黄璐琦;肖培根.
前体化合物和诱导子对丹参酮ⅡA和原儿茶醛合成的优化研究[J]. 中国药学杂志, 2007, 42(24): 1856-1858
GUO Xio-hong;GO Wen-yun;HUNG Lu-qi;XIO Pei-gen.
Optimization Effect of Precursors and Elicitors on Synthesis of Tanshinone ⅡA and Protocatechuic Aldehyde in Adventitious Root Culture of Salvia miltiorrhiza Bge [J]. Chinese Pharmaceutical Journal, 2007, 42(24): 1856-1858
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参考文献
[1] ZHEN G X，FEI Z K. Studies on chemical compounds in roots of Salvia miltiorrhiza[J] .Chin Pharm J（中国药学杂志），1989，24(1)：6-10.
[2] MIRJALILI N，LINDEN J C. Methyl jasmonate induced production of taxol in suspension cultures of Taxus cuspidate：ethylene interation and induction models[J] . Biotechnol Prog，1996，12(1)：110-118.
[3] CHEN H，CHEN F. Effects of yeast elicitor on the growth and secondary metabolism of a high-tanshinone-producing line of the Ti-transformed Salvia miltiorrhiza cells in suspension culture[J] . Process Biochem，2000，35(8)： 837-840.
[4] GUO X H，GAO W Y，CHEN H X，et al. Effects of metal ion on the accumulation o f tanshinone ⅡA and protocatechuic aldehyde in adventitious culture of Salvia miltiorrhiza [J] .China J Chin Mater Med（中国中药杂志），2005，30（12）：885-888.
[5] CHEN H，CHEN F，FRANCIS C K，et al. The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza[J] .Enzyme Microb Technol，2001，28：100-105.
[6] KLESSING D F，MALAMY J. The salicylic acid signal in plants [J] .Plant Mol Biol，1994，26：1439-1458.
[7] CHEN W. Studies on the tissue culture in Salvia miltiorrhiza Bge and Glycyrrhiza uralensis Fisch [D] .Tianjin：Tianjin University，2005.
[8] CHANG J. Effects of La(NO3)3 on root cell membrane penetration in rice and wheat and nutrition elements accumulation[J] . Plant Physiol（植物生理学通讯），1991，27（1）：17-19.
[9] MIAO Z Q，WEI Z J，YUAN Y J. Study on the effects of salicylic acid on taxol biosynthesis.Chinese[J] .Chin J Biotechnol（生物工程学报），2000，16(4)：509-513.
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脚注
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基金
国家863项目(2003AA2Z2040)
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