目的 研究建立小活络丸中掺伪的显微鉴别方法,并为此类相似粉末在中成药中掺伪情况的判定提供依据。方法 采用光学显微镜和台式扫描电镜对小麦粉、大麦粉、青稞粉、燕麦粉进行显微鉴别,并与存在质量问题的小活络丸进行比对。结果 小麦粉、大麦粉、青稞粉及燕麦粉在果皮细胞、横细胞、糊粉层细胞、淀粉粒等特征上存在明显的差别。小活络丸在符合2020年版《中国药典》显微鉴别规定的同时,还观察到了淀粉粒、糊粉层细胞、非腺毛等特征,且与小麦粉的特征较为接近。结论 存在质量问题的小活络丸中掺有小麦粉的可能性较大。本研究为中成药打假的研究提供了思路参考,也为其他补充检验方法的建立奠定了基础。
Abstract
OBJECTIVE To establish a microscopic examination method for the identification of adulteration in Xiaohuoluo pills, and provide identification basis for the adulteration of this kind of similar powder in Chinese patent medicine. METHODS The powder of the dried fruits of Triticum aestivum L., Hordeum vulgare L., Hordeum vulgare L. var. nudum Hook. f., and Avena sativa L. have been identified by optical microscope and desktop scanning electron microscope, and compared with Xiaohuoluo pill with quality problems. RESULTS There are obviously differences in pericarp cells, transverse cells, aleurone layer cells and starch granules between the powder of the dried fruits of Triticum aestivum L., Hordeum vulgare L., Hordeum vulgare L. var. nudum Hook. f., Avena sativa L. While meeting the microscopic identification requirements of the Chinese Pharmacopoeia, Xiaohuoluo pill also observed the starch granules, aleurone layer cells and non-glandular hairs, and it is close to the characteristics of the powder of the dried fruits of Triticum aestivum L. CONCLUSION Xiaohuoluo pills with quality problems are more likely to be mixed with the powder of the dried fruits of Triticum aestivum L.. It can provide ideas and references for the research of anti-counterfeiting of Chinese patent medicine, and also lay a foundation for the establishment of other supplementary test methods.
关键词
小麦 /
小活络丸 /
粉末 /
掺伪 /
台式扫描电镜
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Key words
Triticum aestivum L. /
Xiaohuoluo pill /
powder /
adulteration /
desktop scanning electron microscope
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中图分类号：
R282
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参考文献
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脚注
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基金
国家药品监督管理局中药质量安全与评价重点实验室开放课题资助项目(NIFDC-HT-202000577)
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