目的 建立甘松挥发油指纹图谱,对不同炮制方式的甘松进行质量评价,为甘松的炮制方式提供参考。方法 收集10批次甘松,不同炮制方法处理后,水蒸气蒸馏法提取挥发油。气相色谱法(GC)建立指纹图谱,并采用高分辨质谱对共有峰成分进行鉴定,再以聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)法进行分析,找出3种炮制方式下的主要差异成分。结果 建立了甘松挥发油去土处理、水洗晾干、水洗烘干的GC指纹图谱,共标定18个共有峰,采用化学计量学分析方法可以对不同炮制方式的甘松进行有效区分,高分辨气质联用技术对共有峰成分进行指认,发现不同的炮制方式会对甘松中白菖烯,喇叭烯氯化物-(Ⅱ),百秋李醇,(-)-马兜铃烯等成分影响显著。结论 本实验所建立的甘松挥发油指纹图谱方法精密度、重复性、稳定性良好,且各峰分离度较高,结合化学计量学模式发现不同的炮制方式会对甘松质量产生较大影响,可以为甘松的炮制方式与质量控制提供参考和依据。
Abstract
OBJECTIVE To establish fingerprint method of the volatile oil of Nardostachys jatamansi DC, evaluate the quality of pineapple pine by different processing methods, and analyze the chemical components of the common peaks of Nardostachys jatamansi DC volatile oil by GC-Q-Exactive Orbitrap MS technology. METHODS Ten batches of Nardostachys jatamansi DC were collected and treated with different processing methods, and the volatile oil was extracted by steam distillation. The fingerprint mapping was established by gas chromatography (GC), and the common peak components were identified by high resolution mass spectrometry. The results were analyzed by hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA) to find out the main differences among the three processing methods. RESULTS The results showed that there were 18 common peaks in the GC fingerprint of Nardostachys jatamansi DC volatile oil treated with soil removal, washed with oven dvied. After chemometric analysis, it was found that the medicinal materials under different processing methods could be clearly distinguished.The components of the common peaks were identified by GC-Q-Exactive Orbitrap MS. Different processing methods of Nardostachys jatamansi DC could be effectively distinguished by chemometrical analysis, and it was found that different processing methods could identify the common peaks of (+)-calarene、 patchouli alcohol、(-)-aristolene、 and other components were significant. CONCLUSION The fingerprint of Nardostachys jatamansi DC volatile oil established in this experiment has good precision, repeatability, stability and high resolution of each peak. Furthermore, combined with chemical pattern recognition, different processing methods will have a great impact on the quality of Nardostachys jatamansi DC. The method established in this experiment can provide reference and basis for the processing method and quality control of Nardostachys jatamansi DC.
关键词
甘松挥发油 /
指纹图谱 /
化学计量学分析 /
高分辨质谱
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Key words
Nardostachys jatamansi DC essential oil /
fingerprint /
chemometric analysis /
high-resolution gas-mass spectrometry
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中图分类号：
R282
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脚注
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基金
山东省重点研发计划(重大科技创新工程)项目资助(2021SFGC1202);山东省人文社会科学课题资助(2021-YYGL-44);山东省中药材和饮片标准修订项目资助(2020-061)
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