目的 研究何首乌炮制过程中产生水蒸液的化学成分。方法 使用高效液相色谱对水蒸液化学成分进行表征;采用硅胶柱色谱、Sephadex LH-20葡聚糖凝胶柱色谱、制备液相色谱等色谱技术进行分离和纯化,通过理化方法和波谱数据分析鉴定化合物的结构。结果 与生、制首乌色谱图相比,何首乌水蒸液具有明显差异的色谱峰群;从何首乌水蒸液中分离鉴定了13个化合物,分别为:没食子酸(1),5-羟甲基糠醛(2),原儿茶酸(3),对羟基苯甲酸(4),香草酸(5),香草醛(6),对羟基苯甲醛(7),对香豆酸(8),trans-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(9),cis-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(10),松柏醛(11),芥子醛(12),大黄素(13)。其中化合物1~8,11和12为小分子酚酸类成分,化合物9和10为二苯乙烯苷类化合物,化合物13为蒽醌类化合物。结论 化合物1~12均为首次从何首乌炮制水蒸液分离得到,其中对羟基苯甲酸,对羟基苯甲醛,对香豆酸以及trans-2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷为水蒸液的主要化学成分。
Abstract
OBJECTIVE To investigate the chemical components from the steamed water of Polygonum multiflorum Thunb. METHODS The constituents were separated by HPLC. The compounds were isolated by column chromatography with silica gel, Sephadex LH-20, and preparative HPLC. The structures were elucidated by means of spectral data analysis. RESULTS The visible different chromatographic peaks were found in steamed water by comparison with the crude and processed forms of P. multiflorum. In addition, 13 compounds were isolated, and their structures were determined as gallic acid(1), 5-hydroxymethylfuraldehyde(2), protocatechuic acid(3), p-hydroxybenzonic acid (4), vanillic acid (5), vanillin(6), p-hydroxybenzaldehyde (7), p-coumaric acid (8), trans-2, 3, 5, 4′-tetrahydroxy stilbene-2-O-β-D-glucoside(9),cis-2,3,5,4′-tetrahydroxy stilbene-2-O-β-D-glucoside(10), coniferyl aldehyde (11), sinapaldehyde (12), and emodin(13). CONCLUSION Compounds 1-12 are isolated from the steamed water for the first time, p-hydroxybenzonic acid, p-Hydroxybenzaldehyde, p-coumaric acid, and trans-2, 3, 5, 4′-tetrahydroxy stilbene-2-O-β-D-glucoside are the main components in the steamed water of Polygonum multiflorum Thunb.
关键词
何首乌 /
炮制 /
水蒸液 /
高效液相色谱法 /
二苯乙烯苷 /
酚酸化合物
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Key words
Polygonum multiflorum Thunb. /
processing /
steamed water /
HPLC /
stilbene glycoside /
phenolic acid compound
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中图分类号：
R284
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脚注
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基金
国家自然科学基金资助(81903807,81973476);中央高校基本科研业务费资助(2020-JYB-XJSJJ-012,2021-HXFZJJ-013)
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