Research on in Vitro Anti-Inflammatory Mechanism of Gomisin R, the Lignans of Kadsura longipedunculata Finet et Gagnep
QIN Lang1, MO Dan-dan2, CHENG Ya1, LI Ri-lun1, GONG Xiao-mei2, ZHOU Xiao-lei2, TANG Bing-lan2*
1. Department of Basic Medical Science, Guangxi Medical College, Nanning 530023, China; 2. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plant, Nanning 530023, China
Abstract：OBJECTIVE To explore the effects of gomisin R(the lignans from Kadsura longipedunculata Finet et Gagnep.) on inflammatory cell proliferation and inflammatory factor secretion, and evaluate its anti-inflammatory activity and mechanism.METHODS Inflammation model was built by inducing and activating RAW264.7, the macrophage-monocyte lineage of rats, with lipopolysaccharide(LPS), then the cell survival rate of RAW264.7 was examined via MTT, and the half inhibition concentration(IC50) was calculated. The levels of inflammatory factors, such as TNF-α, IL-1β, IL-6 in cell supernatant were tested via ELISA. The mRNA expression levels of cellular TNF-α, IL-1β, IL-6, IκB-α and NF-κB p65, were measured via RT-PCR. And the protein expression levels of cellular IκB-α and NF-κB p65, were measured via Western blot.RESULTS Compared with the LPS model set, gomisin R could notably inhibit the cell proliferation of RAW264.7 induced by LPS, decrease the TNF-α, IL-1β and IL-6 secretion of cells, enhance the mRNA expression levels of IκB-α, which were decreased by LPS, and in the meantime, lower the mRNA expressions of TNF-α, IL-1β, IL-6 and NF-κB p65, which was increased by LPS, and increase the protein expression of IκB-α while reduce that of NF-κB p65, the result of which was highly dependent on dose and the difference was statistically significant(P<0.05).CONCLUSION Gomisin R can inhibit the mRNA and NF-κB p65 protein expressions of TNF-α, IL-1β, IL-6 and NF-κB p65, and meanwhile increase the protein expressions of IκB-α mRNA and IκB-α, thus reducing the release of inflammatory factors such as TNF-α, IL-1β and IL-6, and bringing remarkable anti-inflammatory activity into full play.
覃朗, 莫单丹, 程娅, 李日伦, 龚小妹, 周小雷, 唐炳兰. 小钻木脂素类化合物戈米辛R的体外抗炎机制研究[J]. 中国药学杂志, 2022, 57(18): 1543-1548.
QIN Lang, MO Dan-dan, CHENG Ya, LI Ri-lun, GONG Xiao-mei, ZHOU Xiao-lei, TANG Bing-lan. Research on in Vitro Anti-Inflammatory Mechanism of Gomisin R, the Lignans of Kadsura longipedunculata Finet et Gagnep. Chinese Pharmaceutical Journal, 2022, 57(18): 1543-1548.
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2022, Vol. 57 
