Experimental Study on the Rapid Identification of Deer Antlers and Their Counterfeit Products Based on PCR-Nucleic Acid Test Strips
XU Ning1, SHAO Bo-yu1, YU Wen-ying1, CHI Kai-yue1, MA Yu-he1, MA Qiu-he1, AI Jin-xia1, LI Ming-cheng1,2, GAO Li-jun1*, XIA Wei1*
1. School of Medical Technology, Beihua University, Jilin 132013, China; 2. Innovation Center for Detection Technology on DNA Fingerprinting of Traditional Chinese Medicine, Jilin 132013, China
Abstract：OBJECTIVE To investigate deer antler authenticity by combining polymerase chain reaction(PCR) and nucleic acid test strips.CONCLUSION Genomic DNA of genuine and counterfeit antler samples was extracted by alkali denaturation method, and the optimal conditions for the identification of antler authenticity by PCR-nucleic acid test strips and agarose gel electrophoresis were established by searching for cytochrome C oxidase subunit I(COI) specific sites, designing antler-specific primers with Primer 3.0 software, and exploring the optimal PCR reaction system and conditions. Meanwhile, the accuracy of antler authenticity identification was verified by clone sequencing.RESULTS The results of this experiment showed that two bands appeared on the PCR-nucleic acid test strips for genuine antlers and one band for the negative control and pseudo antlers, which were consistent with the results of agarose gel electrophoresis, and the sensitivity of the test strips was up to 1 ng·μL-1. 5 of the 9 commercially available antler samples were tested for genuine antlers, with a pass rate of 45%.CONCLUSION The PCR-nucleic acid strip assay is simple, rapid and specific, and can visualize the authenticity of deer antlers within a short period of time with stable results.
徐宁, 邵博宇, 于文莹, 迟凯月, 马玉贺, 马秋贺, 艾金霞, 李明成, 高丽君, 夏薇. 基于PCR-核酸试纸条快速鉴定鹿茸及其伪品的实验研究[J]. 中国药学杂志, 2022, 57(18): 1528-1534.
XU Ning, SHAO Bo-yu, YU Wen-ying, CHI Kai-yue, MA Yu-he, MA Qiu-he, AI Jin-xia, LI Ming-cheng, GAO Li-jun, XIA Wei. Experimental Study on the Rapid Identification of Deer Antlers and Their Counterfeit Products Based on PCR-Nucleic Acid Test Strips. Chinese Pharmaceutical Journal, 2022, 57(18): 1528-1534.
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2022, Vol. 57 
