Genetic Variation and Genetic Differentiation of Gentiana straminea Maxim from Qilian Mountains, Gansu Province by Chloroplast DNA Sequence and ISSR Molecular Markers
ZHU Tian-tian1,2,3, ZHANG Ming-hui1, CAO Hou-kang1, MA Xiao-hui1,2, MENG Wei-ye1, JIN Ling1,2,3*
1. Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China; 2. Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-con Structed by Gansu Province MED of PRC, Lanzhou 730000, China; 3. Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the University of Gansu Province, Lanzhou 730000, China
Abstract：OBJECTIVE To analyze the sequence variation and genetic differentiation of Gentiana straminea Maxim. in Qilian mountain, Gansu province, using the chloroplast gene sequence and ISSR molecular marker technology in this experiment. METHODS Total DNA of G. straminea was extracted by kit extraction method, amplified and sequenced by two selected primers, the sequences were corrected and spliced by software Chromas and ContigExpress, the sequence characteristics of spliced sequences were analyzed by software MegaX, DanSP5, and GenALEx, and finally the haplotype network evolution diagram of G. straminea was drawn by software PopART. RESULTS A total of 114 individuals from 18 populations of G. straminea were successfully amplified and sequenced. The splice length of the two chloroplast DNA (cp DNA) fragments was 1 246 bp, and there were 676 polymorphic mutation sites, 459 single mutation sites, 217 condensed information sites, and 107 insert-deletion sites. The number of haplotypes was 41. Tajima′s D* test was not significant at P>0.05, which followed the neutral evolution model as a whole. The percentage of variation within the population was 89%, indicating that genetic variation mainly existed within the populations. The estimated pair-wise genetic differentiation (Fst) among the populations is 0.114 (P<0.05), and the gene flow was 3.889, respectively, indicating low genetic differentiation and a relatively high level of gene flow among populations. There was no obvious correlation between genetic differentiation among populations and geographical distance(r=-0.094, P<0.05). CONCLUSION The results show that G. straminea in the Qilian mountain area of Gansu province had high genetic diversity at the species level and rich haplotype types. The genetic variation of the population mainly come from genetic drift.
朱田田, 张明惠, 曹后康, 马晓辉, 孟伟业, 晋玲. 基于cp DNA序列和ISSR分子标记的甘肃祁连山地区麻花秦艽变异与遗传分化分析[J]. 中国药学杂志, 2022, 57(9): 691-699.
ZHU Tian-tian, ZHANG Ming-hui, CAO Hou-kang, MA Xiao-hui, MENG Wei-ye, JIN Ling. Genetic Variation and Genetic Differentiation of Gentiana straminea Maxim from Qilian Mountains, Gansu Province by Chloroplast DNA Sequence and ISSR Molecular Markers. Chinese Pharmaceutical Journal, 2022, 57(9): 691-699.
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