Abstract：By summarizing the research progress of the existing in vitro pharmacology research METHODS of traditional Chinese medicine(TCM) -medicated serum method, medicated cerebrospinal solution method and medicated intestinal absorption solution method, the advantages and disadvantages of each method were compared, to provide a reference for the discussion of new METHODS for more reliable in vitro pharmacology research of TCM. This article puts forward ideas for the improvement of medicated intestinal absorption solution method, and a new method of medicated liver incubation solution method was established to play a role in inducing more scientific in vitro pharmacological METHODS of TCM. It hopes to provide reference for the exploration of in vitro pharmacological experimental METHODS of TCM, and promote the development of multi-mode research on the pharmacodynamic substances and mechanism of TCM.
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