Abstract：OBJECTIVE To investigate the relationship between the recovery of puerarin on self-made linear microdialysis probes and the perfusion speed and drug concentrations for further ocular pharmacokinetic study. METHODS The concentration of puerarin in microdialysate was determined by HPLC. The in vitro recovery of puerarin was studied using positive dialysis and retrodialysis methods at different flow rates and concentrations, respectively. And the in vivo recovery of puerarin was studied using retrodialysis methods. RESULTS Within the range of drug concentration and perfusion rate studied, the recovery rate of puerarin on microdialysis probe was independent of drug concentration and inversely proportional to perfusion rate. There was no significant difference between the positive dialysis and retrodialysis recovery in vitro at the perfusion flow rate of 1.0、2.0 or 3.0 μL·min-1 (P>0.05), the retrodialysis recovery in vivo was significantly lower than those of in vitro (P<0.05). CONCLUSION The recovery rate of the self-made linear probe is stable, and the microdialysis technique can be used for the study of the ocular pharmacokinetics of puerarin.
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2022, Vol. 57 
