湖南中医药大学, a. 中医药防治眼耳鼻咽喉疾病湖南省重点实验室, b. 医学院, c. 湘产大宗药材品质评价湖南省重点实验室, 长沙 410208
The Effect of Berberine Combined with Ginsenoside Rg3 on Xenograft Model of Nasopharyngeal Carcinoma
ZHOU Fang-lianga,b,c, LIN Tinga, LIU Jiea, LUO Jing-jinga,b, HE Ying-chuna,b*, LIAO Duan-fangc
a. Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, b. College of Medicine, c. Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province State, Hunan University of Chinese Medicine, Changsha 410208, China
Abstract：OBJECTIVE To study the effect and possible mechanism of berberine combined with ginsenoside Rg3 on transplanted tumor of nasopharyngeal carcinoma. METHODS The antitumor effect of berberine or/and ginsenoside Rg3 were observed after establishing xenograft model of nasopharyngeal carcinoma. The effect of drugs on the apoptosis of tumor cells in vivo was examined by Tunel staining. Western blot and immunohistochemistry were used to examine the effects of berberine or/and ginsenoside Rg3 on the expressions of cell proliferation-, apoptosis-related proteins and key proteins of PI3K/AKT and MAPK/ERK in vivo. RESULTS Compared with the solvent control group, the weight of transplanted tumor in Berberine group, Ginsenoside Rg3 group, cisplatin group and combination group decreased significantly, while the apoptotic index was increased, and the effect of combined treatment group was better than that of two single drug groups. Compared with single drug group, the expressions of Survivin, PCNA,Bcl-2, p-AKT and p-ERK were down-regulated, while the expressions of Bax was up-regulated in the combined group (P<0.05). CONCLUSION Either single or combined use of berberine and ginsenoside Rg3 can synergistically inhibit the proliferation and induce cell apoptosis of NPC CNE2 cells in vivo. The mechanism is related to PI3K / Akt and MAPK / ERK signaling pathways.
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ZHOU Fang-liang, LIN Ting, LIU Jie, LUO Jing-jing, HE Ying-chun, LIAO Duan-fang. The Effect of Berberine Combined with Ginsenoside Rg3 on Xenograft Model of Nasopharyngeal Carcinoma. Chinese Pharmaceutical Journal, 2021, 56(18): 1496-1502.
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2021, Vol. 56 
