Abstract：OBJECTIVE To investigate the effect of Boschniakia rossica polysaccharides (BRPS) on the inflammatory response of RAW264.7 macrophages induced by lipopolysaccharide (LPS). METHODS The cellular model was established by treating RAW264.7 macrophages with LPS. The macrophages were randomly assigned into normal group, model group, BRPS low-, medium- and high-dose groups (the final concentrations of BRPS were 25, 50 and 100 mg·L-1, respectively). Cell viability was detected by the cell counting kit-8 (CCK-8); the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured with enzyme-linked immunosorbent assay (ELISA); the protein expressions of cyclooxygenase-2 (COX-2), myeloid differentiation factor 88 (MyD88) and interleukin-1 receptor-associated kinase1 (IRAK1), and the activation of nuclear factor-кB (NF-кB), mitogen activated protein kinases (MAPK) and signal transducers and activators of transcription-3 (STAT3) were determined with Western blot method. RESULTS LPS had no significant effect on the viabilities of RAW264.7 macrophages in the concentration range of 100-2 000 μg·L-1, but could significantly up-regulate the expression of COX-2 protein in RAW264.7 cells, indicating the establishment of a macrophage model of inflammation. BRPS had no significant effect on the viabilities of RAW264.7 cells in the concentration range of 0-100 mg·L-1, however, it could down-regulate the LPS-induced secretions of IL-1β, IL-6 and TNF-α into the culture media of RAW264.7 cells. Compared with the model group, the inhibition ratios of IL-1β secretion were 14.2%, 47.6% and 72.4%; inhibition ratios of IL-6 secretion were 5.5%, 22.2% and 45.1%; and inhibition ratios of TNF-α secretion were 9.5%, 29.1% and 55.3% in BRPS low-, medium- and high-dose groups, respectively. At the same time, BRPS could reduce the LPS-induced COX-2 protein expression, down-regulate the expressions of MyD88 and IRAK1 proteins, inhibit the phosphorylation and nuclear translocation of NF-кB p65, and inhibit the phosphorylation of ERK, JNK, p38 and STAT3. CONCLUSION BRPS has an inhibitory effect on LPS-induced RAW264.7 macrophage inflammatory response, maybe through the suppression of NF-кB, MAPK, and STAT3 activation.
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2021, Vol. 56 
