Abstract：OBJECTIVE To investigate the effect and mechanism of oridonin on MPP+-induced SK-N-SH cell damage. METHODS The experiment was divided into control group, MPP+ group, oridonin low, medium, and high concentration groups, pcDNA group, pcDNA-LINC00707 group, si-NC group, si-LINC00707 group, MPP++si-NC group, MPP++si-LINC00707 group, MPP++oridonin+pcDNA group, and MPP++oridonin+pcDNA-LINC00707 group. Flow cytometry was used to detect apoptosis; Western blot was used to detect Bcl-2 and Bax protein expression; lactate dehydrogenase (LDH) kit was used to detect LDH release in cell supernatant; malondialdehyde (MDA) kit and glutathione (GSH) kit were used to detect MDA and GSH content in cells; real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expressions of LINC00707 and miR-145-5p; dual luciferase reporter assay was used to detect the targeting relationship between LINC00707 and miR-145-5p. RESULTS After treatment with oridonin at low, medium and high concentrations, the MDA content in SK-N-SH cells induced by MPP+ was significantly reduced, the apoptosis rate was significantly reduced, and the Bcl-2 expression was significantly increased, the expression of Bax was significantly reduced, the LDH level was increased, the MDA content was significantly reduced, and the GSH content was significantly increased, the expression of LINC00707 was significantly decreased, and the expression of miR-145-5p was significantly and concentration-dependently increased (P<0.05). LINC00707 targets miR-145-5p and inhibited the expression of LINC00707, MPP+-induced apoptosis and oxidative stress in SK-N-SH cells; overexpression of LINC00707 reversed the protective effect of oridonin on MPP+-induced SK-N-SH cell injury. CONCLUSION Oridonin can inhibit MPP+-induced apoptosis and oxidative stress in SK-N-SH cells, and its mechanism may be related to LINC00707 and miR-145-5p.
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2021, Vol. 56 
