Evaluation of Chemical Liver Injury Based on Metabolic Profiling of Serum Fatty Acids
LIU Xiao-jiea, LI Hong-weia, HU Conga, WU Lin-jinga, XIONG Yin-huaa,b*
a. School of Pharmacy; b. Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Science and Technology Normal University, Nanchang 330013, China
Abstract：OBJECTIVE To evaluate the chemical liver injury induced by carbon tetrachloride (CCl4) and α-naphthalene isothiocyanate (ANIT). METHODS The serum concentrations of 15 kinds of free and esterified fatty acids in rats in CCl4 control group, CCl4 treatment group, ANIT control group and ANIT treatment group were determined by gas chromatography-mass spectrometry. DB-225MS capillary column was used with programmed temperature increase, and electron impact ion source of 70 eV and selective ion scanning mode (monitoring at m/z 55, 67, 74, 79) were used. The metabolic profiles of serum fatty acids in rats after liver injury were measured by chemometrics such as partial least squares-discriminant analysis and principal component analysis. Pearson and canonical correlation analyses were used to analyze the relationship between chemical liver injury and fatty acid metabolism. RESULTS The metabolic profiles of fatty acids in the serum of CCl4-treated and ANIT-treated rats obviously deviated from those of the control rats, and could be completely distinguished from each other. Linoleic acid, docosahexaenoic acid, oleic acid, arachidonic acid and stearic acid played an important role in the chemical liver injury induced by CCl4 and ANIT. Furthermore, these five free fatty acids were positively correlated with aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase (P<0.05, P<0.01). CONCLUSION The metabolic profile of serum fatty acids is closely related to CCl4-induced and ANIT-induced chemical liver injuries.
刘晓杰, 黎红维, 胡聪, 吴琳静, 熊印华. 基于血清脂肪酸代谢轮廓的化学肝损伤评价研究[J]. 中国药学杂志, 2021, 56(8): 647-653.
LIU Xiao-jie, LI Hong-wei, HU Cong, WU Lin-jing, XIONG Yin-hua. Evaluation of Chemical Liver Injury Based on Metabolic Profiling of Serum Fatty Acids. Chinese Pharmaceutical Journal, 2021, 56(8): 647-653.
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2021, Vol. 56 
