Effects of Nitrogen Forms on Main Phytochemical Content and Antioxidant Activity in Root Tubers of Tetrastigma hemsleyanum
FU Li-zhong1, ZHAO Li-mei1, LIU Qian1, FENG Yue1, KAI Guo-yin1, LU Ting-gao2
1. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China; 2. Hangzhou Fuyang Jinhulu Ecological and Agricultural Development Co., Ltd, Hangzhou 310012, China
Abstract：OBJECTIVE To study the effects of nitrogen form on main phytochemical content and antioxidant activity of root tubers of T. hemsleyanum at the same nitrogen level, and provide the basis for the scientific application of nitrogen in the artificial cultivation of Tetrastigma hemsleyanum. METHODS Container experiments were carried out in the green house. Five ratios of NH4+-N/NO3--N (0∶100, 25∶75, 50∶50, 75∶25, 100∶0), amide nitrogen and no nitrogen were set up, and randomized block design with three replications was used. After two years of nitrogen application, the polysaccharides, total flavonoids and phenols, and antioxidative activities (ABTS and FRAP) in root tubers of T. hemsleyanum were determined, and variance analysis, correlation analysis, cluster analysis and principal component analysis were employed. RESULTS The result showed that the nitrogen form had little effect on the polysaccharide content, but had great effects on the total flavonoids and phenols content, and antioxidant activity. With the increase of the ratio of ammonium nitrogen, total flavonoids and phenols content and antioxidant activity of root tubers decreased significantly, and rise sharply when NH4+-N/NO3--N is 75∶25, and reach the maximum when NH4+-N/NO3--N is 100∶0. The total flavonoids and phenols content and antioxidant activity in the treatment of ammonium nitrogen were significantly higher than those in the treatment of amide nitrogen, nitrate nitrogen and no nitrogen. CONCLUSION These results indicate that NH4+-N/NO3--N is 75∶25 and 100∶0, it is more beneficial to the accumulation of total flavonoids and phenols, and the improvement of antioxidant activity in root tubers of T. hemsleyanum.
付立忠, 赵利梅, 刘骞, 冯岳, 开国银, 卢廷高. 氮素形态对三叶青块根主要化学成分含量及抗氧化活性的影响[J]. 中国药学杂志, 2021, 56(8): 633-639.
FU Li-zhong, ZHAO Li-mei, LIU Qian, FENG Yue, KAI Guo-yin, LU Ting-gao. Effects of Nitrogen Forms on Main Phytochemical Content and Antioxidant Activity in Root Tubers of Tetrastigma hemsleyanum. Chinese Pharmaceutical Journal, 2021, 56(8): 633-639.
WANG C Y, LEE J S, SU X D, et al. Three new constituents from the aerial parts of Tetrastigma hemsleyanum[J]. Phytochem Lett, 2018, 27: 25-29.
[2]
Zhejiang Food and Drug Administration. Processing Standard of Traditional Chinese Medicine in Zhejiang(2015)(浙江省中药炮制规范2015年版)[M]. Beijing:Chinese Medicine Science and Technology Publishing House, 2015:5-6.
[3]
LIN J, JI M M, HUANG Z H, et al. Chemical constituents of whole plants of Tetrastigma hemsleyanum and their antitumor activities[J]. Chin Pharm J(中国药学杂志), 2015, 50(8):658-663.
[4]
DING L, ZHANG L X, QIU Y, et al. Chemical constituents in chloroform extraction of Tetrastigmatis hemsleyani Diels et. Gilg and their antitumor activities[J]. Chin Pharm J(中国药学杂志), 2015, 50(21):1857-1860.
[5]
XU C J, DING G Q, FU J Y, et al. Immunoregulatory effects of ethyl-acetate fraction of extracts from Tetrastigma hemsleyanum Diels et Gilg on immune functions of ICR mice[J]. Biomed Environ Sci, 2008, 21(4): 325-331.
[6]
CHU Q, JIA R, CHEN M, et al. Tetrastigma hemsleyanum tubers polysaccharide ameliorates LPS-induced inflammation in macrophages and Caenorhabditis elegans[J]. Int J Biol Macromol, 2019, 141: 611-621.
[7]
CHEN X, TAO L, RU Y, et al. Antibacterial mechanism of Tetrastigma hemsleyanum Diels et Gilg′s polysaccharides by metabolomics based on HPLC/MS[J]. Int J Biol Macromol, 2019, 140: 206-215.
[8]
CHENG L S. Biological characteristics and cultivation techniques under forest of medicinal plant Tetrastigma hemsleyanum[J]. Mod Agri Sci Technol(现代农业科技), 2014, (14):187-188.
[9]
FU L Z, ZHAO L M, LV H Q, et al. Effects of nitrogen level on growth of Tetrastigma hemsleyanum and phytochemical content and antioxidant activity in stems and leaves[J]. China J Chin Mater Med(中国中药杂志), 2019, 44(4):696-702.
[10]
ZHANG Q, LIU Y, WU X M, et al. Preliminary study on the effects of two different nitrogen forms on flavonoids accumulation and related molecular mechanism in medicinal plant Tetrastigma hemsleyanum[J]. Bull Bot Res(植物研究), 2018, 38(3):367-376.
[11]
INOKUEHI R, KUMA K, MIYATA T, et al. Nitrogen assimilating enzymes in land plants and algae: phylogenic and physiological perspectives[J]. Physiol Plantarum, 2002, 116(1):1-11.
[12]
IBRAHIM M H, JAAFAR H Z E, RAHMAT A, et al. The relationship between phenolics and flavonoids production with total non structural carbohydrate and photosynthetic rate in Labisia pumila Benth. under high CO2 and nitrogen fertilization[J]. Molecules, 2011, 16(1):162-174.
[13]
JIN L, LI L B, TIAN D Q, et al. Antioxidant properties and color parameters of herbal teas in China[J]. Ind Crop Prod, 2016, 87: 198-209.
[14]
TANG Q Y, ZHANG C X. Data processing system (DPS) software with experimental design, statistical analysis and data mining developed for use in entomological research[J]. Insect Sci, 2013, 20(2): 254-260.
[15]
TANG Q Y. DPS Data Processing System: Expermental Design, Statistical Analysis and Data Mining(2nd Edition) [DPS数据处理系统:实验设计、统计分析及数据挖掘(第2版)] [M]. Beijing: Science Press, 2010:761-771.
[16]
MALAGOLI M, CANAL D, QUAGGIOTTI S. Differences in nitrate and ammonium uptake between Scots pine and European lacrh[J]. Plant Soil, 2000, 221(1):1-3.
[17]
ZHENG J, CAO F L, YU W W. Leaf quality of Ginkgo biloba at the late growing stage with different NO-3-N and NH+4-N combinations[J]. J Zhejiang Forest Coll(浙江林学院学报), 2007, 24(5):564-568.
[18]
ZHU Z B, YU M M, CHEN Y H, et al. Effects of ammonium to nitrate ratio on growth, nitrogen metabolism, photosynthetic efficiency and bioactive phytochemical production of Prunella vulgaris[J]. Pharm Biol, 2014, 52(12): 1518-1525.
[19]
ZHANG P, WANG K C, ZHAO J, et al. Effects of NH+4-N/NO-3-N ratio on secondary metabolism and disease resistance of Chrysanthemum morifolium[J]. J Plant Nutr Fertil(植物营养与肥料学报), 2014, 20(6):1488-1496.
[20]
KOVACIK J, KLEJDUS B. Induction of phenolic metabolitesand physiological changes in chamomile plants in relation to nitrogen nutrition[J]. Food Chem, 2014, 142: 334-341.
[21]
ZHU W F. Effects of nitrogen nutrition on antioxidant contents and antioxidant activities in Chinese cabbage (Brassica chinensis L.)[D]. Hangzhou: Zhejiang University, 2008.
[22]
LU L L, YANG X Q, WANG C X, et al. Effects of nitrogen supply with different NO-3/NH+4 ratios on growth and medicinal components of Pogostemon cablin[J]. J Plant Nutri Fertil(植物营养与肥料学报), 2017, 23(5):1314-1325.
[23]
YE C L, LIU X G. Extraction of flavonoids from Tetrastigma hemsleyanum Diels et Gilg and their antioxidant activity[J]. J Food Proc Presc, 2015, 39(6):2197-2205.
[24]
SUN Y, TSAO R, CHEN F, et al. The phenolic profiles of Radix Tetrastigma after solid phase extraction (SPE) and their antitumor effects and antioxidant activities in H22 tumor-bearing mice[J]. Food Funct, 2017, 8(11):4014-4027.
[25]
SUN Y, LI H Y, HU J N, et al. Qualitative and quantitative analysis of phenolics in Tetrastigma hemsleyanum and their antioxidant and antiproliferative activities[J]. J Agric Food Chem, 2013, 61(44):10507-10515.
2021, Vol. 56 
