Preparation of Realgar Nanoparticles and Apoptosis of A549 Cells Induced by Targeted Survivin Protein
WANG Sheng-mei1, LIU Xuan-jun2, DENG Gui-ming1*, HE Ya-na1
1. The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China; 2. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China
Abstract：OBJECTIVE To prepare realgar nanoparticles and investigate their physicochemical properties, study the effect of realgar nanoparticles on the proliferation and apoptosis of human lung cancer cell A549. METHODS Using polyvinylpyrrolidone K30 (PVP) as a carrier, realgar nanoparticles were prepared by the biological template method. The prepared nanoparticles were characterized by transmission electron microscope (transmission electron microscope,TEM), infrared spectroscopy, ultraviolet spectroscopy and particle size analysis, and the drug loading and entrapment efficiency of As4S4 in the nanoparticles were determined by atomic absorption spectrometry. The inhibitory activity of realgar nanoparticles on A549 cells in vitro was detected by thiazolyl (MTT) method, and the half inhibitory concentration (IC50) was calculated. The apoptosis of A549 cells was detected by Annexin V/PI double staining, and the expression of survivin protein was detected by Western blot. RESULTS The prepared realgar nanoparticles are spherical particles with regular morphology and uniform dispersion. The size of the nanoparticles is about 50.7 nm and the potential is -23.3 mV. After freeze-drying and reconstitution, the size of the nanoparticles is about 68.1 nm and the potential is -21.4 mV, the encapsulation rate of As4S4@PVP NPs is 25.9%, and the drug loading is 13.4%. The ultraviolet spectrum shows that the characteristic absorption peak of PVP appears in As4S4@PVP NPs, and the infrared spectrum analysis shows the infrared spectrum peaks of As4S4@PVP NPs and PVP. The position of As4S4@PVP NPs did not change significantly within 48 h under the conditions of H2O, PBS, and RPMI-1640 complete media. The in vitro results showed that nano realgar can significantly inhibit the proliferation of A549 cells. Compared with the control group, As4S4 @PVP NPs can effectively induce cell apoptosis and reduce the expression of survivin protein. CONCLUSION Realgar nanoparticles are successfully prepared, and the size of the nanoparticles is small and stable, which could effectively induce apoptosis of lung cancer A549 cells by reducing the expression of survivin.
王胜玫, 刘炫均, 邓桂明, 何娅娜. 雄黄纳米粒的制备及其靶向Survivin蛋白诱导A549细胞凋亡的研究[J]. 中国药学杂志, 2021, 56(7): 553-558.
WANG Sheng-mei, LIU Xuan-jun, DENG Gui-ming, HE Ya-na. Preparation of Realgar Nanoparticles and Apoptosis of A549 Cells Induced by Targeted Survivin Protein. Chinese Pharmaceutical Journal, 2021, 56(7): 553-558.
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2021, Vol. 56 
