大理大学, a. 药用特种昆虫开发国家地方联合工程研究中心; b. 云南省昆虫生物医药研发重点实验室; c. 中国西南药用昆虫及蛛形类资源开发利用2011协同创新中心, 云南 大理 671000
Composition Analysis and Biological Activity Evaluation of the Venom of Vespa basalis (Smith)
YUAN Shi-menga,b, CHE Yi-haoa,b, YANG Zhi-bina,b,c, YANG Da-songa,b,c, XIAO Huaia,b,c, ZHAO Hai-ronga,b,c, ZHANG Cheng-guia,b,c, HE Miaoa,b,c*, WU Xiu-Meia,b,c*
a. National-Local Joint Engineering Research Center of Entomoceutics; b. Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D; c. Yunnan Provincial 2011 Collaborative Innovation Center for Ento-moceutics, Dali University, Dali 671000, China
Abstract：OBJECTIVE To analyze the composition of Vespa basalis (Smith) and evaluate its biological activity in vitro. METHODS Using SDS-PAGE and UPLC-ESI-Q-TOF-MS to analyze the relative molecular mass composition of proteins and peptides in the venom. The MTT method was used to evaluate the anti-inflammatory and anti-tumor activities and the Oxford Cup method was used to study the antibacterial effect of the venom. In addition, the results were compared with the ethanol extract of the Vespa basalis adult. RESULTS The proteins in the venom was mainly distributed in the range of 11-48×103, and there were 3 more obvious protein bands around 26, 34 and 43×103 respectively,which were the main allergen components of wasp venom. The relative molecular masses of peptides presented an irregular multimodal distribution and 85% were concentrated in the 300-2 500 range. In terms of biological activity, Vespa basalis venom had an inhibitory effect on the proliferation of RAW264.7 cells induced by lipopolysaccharide LPS. The inhibitory rate of 25 μg·mL-1 venom was 67.67%, and the anti-inflammatory activity of 100 μg·mL-1 was 10 times that of dexamethasone with the same concentration. It had significant killing effect on the growth breeding of HT-29, HepG2, and B16 tumor cells. In addition, it exhibited a growth inhibitory effect on Staphylococcus aureus, Escherichia coli and Candida albicans. While the ethanol extract only showed an inhibitory activity on the proliferation of induced RAW264.7 cells, and the inhibitory effect was only about 1/60 of venom. CONCLUSION This experiment used modern separation and analysis techniques to conduct a relatively systematic study on the composition and biological activity of Vespa basalis venom. From the results, it can be seen that venom is rich in peptides substances, which provided a direction for the selection of follow-up research methods of the venom; In addition to the anti-inflammatory activity, Vespa basalis venom also had anti-tumor and antibacterial effects, indicating that it has potential medicinal purposes development value, and which provided a reference for subsequent in-depth research on the modernization of this venom.
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