Geoherbalism and Grand Evaluation of Zhejiang Ophiopogon japonicas Based on PCA and PLS-DA
HE Jia1, HUANG Wen-kang4, MA Xiang-feng5, MA Lin-ke2,3, WU Jun5, YE Li-hua5, CHEN Bi-lian2,3, LIU Li5*, ZHANG Wen-ting2,3*
1. College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China; 2. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China; 3. State Drug Administration Key Laboratory for Quality Evaluation of Proprietary Chinese Medicines, Hangzhou 310052, China; 4. Zhejiang Academy of Medical Sciences, Hangzhou 310015, China; 5. Zhengda Qingchunbao Pharmaceutical Limited Company, Hangzhou 310023, China
Abstract：OBJECTIVE To explore the geoherbalism and grand evaluation of Zhejiang Ophiopogon japonicas. METHODS A total of 42 batches of Zhejiang Ophiopogon japonicas samples were collected from Cixi city and Sanmen county, Zhejiang province. The samples were divided into three grades according to the number of grains per 50 g. HPLC method was used to determine the contents of seven components in Zhejiang Ophiopogon japonicas and evaluate the geoherbalism of samples according to the PCA synthesis score. PLS-DA was used to analyze the classification by combining appearance and intrinsic chemical content. RESULTS The result of PCA(principal component analysis) synthesis score showed that the samples from Cixi city was better than the samples from Sanmen city. The PLS-DA results showed that 42 batches of samples could be clearly divided into three categories, among which the first-class samples were obviously clustered into one category, the second-class and third-class samples from Cixi city were clustered into one category, and the second-class and third-class samples from Sanmen county were clustered into one category. Combined with appearance and internal indicators, there are five indicators that can be used as classification markers. CONCLUSION The traditional grading evaluation method has some limitations. Evaluation of the rank of Zhejiang Ophiopogon japonicus by combining the traditional appearance index and internal content index can get scientific result, which provides a new idea for the rank evaluation of Zhejiang Ophiopogon japonicas.
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HE Jia, HUANG Wen-kang, MA Xiang-feng, MA Lin-ke, WU Jun, YE Li-hua, CHEN Bi-lian, LIU Li, ZHANG Wen-ting. Geoherbalism and Grand Evaluation of Zhejiang Ophiopogon japonicas Based on PCA and PLS-DA. Chinese Pharmaceutical Journal, 2021, 56(4): 285-292.
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2021, Vol. 56 
