1.三峡大学, a. 生物与制药学院; b. 国家中医药管理局中医药科研三级实验室, 湖北 宜昌 443002; 2.湖北民康制药有限公司, 湖北 宜昌 443002
Determination of Five Triterpenes in Centellae Herba by A Combinative Method of HPLC Fingerprint and Quantitative Analysis of Multi-Components by Single Marker
XU Hai-yan1a,b, HE Chun-xi1a,b, LUO Si-xu1a, YANG Zhen-qing2, HE Hai-bo1b*, HE Yu-min1a*
1a. College of Biological and Pharmaceutical Sciences; 1b. Third-Grade Pharmacological Laboratory on Traditional Chinese Medicine Approved by State Administration of Traditional Chinese Medicine, China Three Gorges University, Yichang 443002, China; 2. Hubei Minkang Pharmaceutical Co., Ltd., Yichang 443002, China
Abstract：OBJECTIVE To establish a method for simultaneous determination of five typical triterpenes in Centellae Herba by a combinative method of HPLC fingerprint and quantitative analysis of multi-components by single marker (QAMS), and verify its applicability and accuracy in the quality control of Centellae Herba. METHODS The HPLC fingerprint analysis method for Centellae Herba was developed. Based on this, QAMS method was developed and evaluated by comparison of the quantitative results with the external standard method. RESULTS The HPLC fingerprints of Centellae Herba were established using 12 batches of samples. Twenty-three peaks were acquired as co-existing peaks and eight peaks among them were identified with chemical references. With asiaticoside as internal reference substance, QAMS was developed and the mean fs/k of asiaticoside B, madecassoside, madecassic acid and asiatic acid were 0.85, 1.13, 0.46 and 0.45, respectively. The contents of four triterpenes in 12 batches of Centellae Herba measured by QAMS method were not significantly different from those using the external standard method. CONCLUSION The established method is simple, accurate and reliable, which can be used for the quality evaluation of five triterpenes in Centellae Herba.
许海燕, 何春喜, 罗思旭, 杨振清, 贺海波, 何毓敏. 指纹图谱与一测多评法相结合测定积雪草中5种三萜类成分[J]. 中国药学杂志, 2021, 56(3): 181-188.
XU Hai-yan, HE Chun-xi, LUO Si-xu, YANG Zhen-qing, HE Hai-bo, HE Yu-min. Determination of Five Triterpenes in Centellae Herba by A Combinative Method of HPLC Fingerprint and Quantitative Analysis of Multi-Components by Single Marker. Chinese Pharmaceutical Journal, 2021, 56(3): 181-188.
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2021, Vol. 56 
