Analysis of Brain Components and Mechanism of Danshen Ligustrazine Injection Based on UPLC-Q/TOF-MS and Network Pharmacology
DONG Qing-hai1, LIU Hui2, ZHOU Bai-song1, JIAO Yu-feng1, SI Yu1, LIU Jin-ping1, LI Ping-ya1, LIN Hong-qiang1*
1. Natural Products Research Center, College of Pharmacy, Jilin University, Changchun 130021, China; 2. Jilin Sichang Pharmaceutical Co., Ltd., Meihekou 135000, China
Abstract：OBJECTIVE To establish a method for determination of the components in Danshen Ligustrazine Injection which can penetrate into brain by ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q/TOF-MS), and explore the mechanism of action by network pharmacology. METHODS UPLC-Q/TOF-MS technique was used to qualitatively analyze the prototype components penetrating into the brain of rats. TCMSP, DAVID and other databases were used to predict the targets and biological pathways of the components, and the brain component-target-biological pathway network was constructed by Cytoscape software. RESULTS Seven kinds of components were identified in the brain tissue of rats, involving 98 targets, which acted on 10 pathways including cGMP-PKG signaling pathway and calcium signaling pathway. CONCLUSION UPLC-Q/TOF-MS technique is a rapid and reliable method for analyzing the components penetrating into brain of Danshen Ligustrazine Injection. The mechanism of action and pharmacodynamic substances of the injection for the pharmacological effects in brain were preliminarily clarified by network pharmacology method.
董庆海, 刘慧, 周柏松, 焦玉凤, 司雨, 刘金平, 李平亚, 林红强. 基于UPLC-Q/TOF-MS和网络药理学分析丹参川芎嗪注射液入脑成分及作用机制[J]. 中国药学杂志, 2020, 55(21): 1795-1801.
DONG Qing-hai, LIU Hui, ZHOU Bai-song, JIAO Yu-feng, SI Yu, LIU Jin-ping, LI Ping-ya, LIN Hong-qiang. Analysis of Brain Components and Mechanism of Danshen Ligustrazine Injection Based on UPLC-Q/TOF-MS and Network Pharmacology. Chinese Pharmaceutical Journal, 2020, 55(21): 1795-1801.
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2020, Vol. 55 
