Chemical Constituents from Leonurus japonicus Houtt. and Their Antioxidant Activity
PENG Fanga,b, XIONG Lianga,b, HE Yu-lina,b, ZHU Huana,b, CHEN Dan-dana, GUO Lia,b, LIU Feia,b*, PENG Chenga*
a. College of Pharmcy, Key Laboratory of Standardization of Chinese Herbal Medicine, State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, The Ministry of Education; b. Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
Abstract：OBJECTIVE To study the chemical constituents of Leonurus japonicus Houtt. and their antioxidant activity. METHODS The silica gel, Sephadex LH-20 gel, reversed phase C18 column chromatography, semi-preparative thin layer chromatography and high performance liquid chromatography were used for isolation and purification. The structure of each compound was identified by organic spectroscopy. The antioxidant activity of each compound was tested by the ABTS method and the DPPH method. RESULTS Fourteen compounds were isolated from the n-butanol fraction of L. japonicas, which were izalpinin (1), methyl(E)-3-(2,5-dihydroxyphenyl)acrylate (2), alatusol D (3), 2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (4), 3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone (5), methyl 4-hydroxy-3-methoxybenzoate (6), methyl 4-hydroxy-3,5-dimethoxybenzoate (7), 3,4,5-trimethoxybenzoic acid (8), vanillic acid (9), 4-(methoxymethyl)-phenol (10), 4-hydroxybenzoic acid (11), 4-methoxy-benzoic acid (12), uracil (13), methyl linoleate (14). The antioxidant activities of compounds 1, 3, 4, 8, and 14 were determined by DPPH and ABTS METHODS. CONCLUSION Compounds 1-7, 10, 12 and 14 are isolated from the genus Leonurus for the first time. Compound 1 has good antioxidant activity. Its scavenging effect on ABTS radical cations is better than that of positive drug vitamin C, while the scavenging effect on DPPH free radicals is slightly weaker.
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2020, Vol. 55 
