Optimization of Synthesis of Rehmannia glutinosa Libosch Polysaccharide-Iron(Ⅲ) by Response Surface Method
LIU Chong-ying1, LI Jin1, ZHOU Ning2, CUI Tao3, ZHANG Ting-ting1, PU Ding-tao1, CHEN Yan1, ZHANG Li-hua1, ZHAO Peng1*
1. School of Pharmacy, Shaan Xi University of Chinese Medicine/Key Labortary of Traditional Chinese Medicine Basic and New Drug Research of Shanxi Province, Xianyang 712046, China; 2. Beijing Guorenguan Medical Research Institute, Beijing 100020, China; 3. Herbaceous Source (Tianjin) Medical Management Service Co., Ltd., Tianjin 300131, China
Abstract：OBJECTIVE To synthesize Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) via polysaccharide from Rehmannia glutinosa Libosch. METHODS The synthetic process was optimized via response surface method on the basis of single factor experiment. Its chemical structure was characterized by infrared spectrum (IR) and scanning electron microscope (SEM). Its drug release percentage was determined in simulated gastrointestinal liquid. RESULTS The optimum conditions were as follows: m(ferric chloride): m(Rehmannia glutinosa Libosch polysaccharide)=2.0, m(trisodium citrate): m(Rehmannia glutinosa Libosch polysaccharide)=1.45, 80 ℃, pH 8.0, and reaction time of 1.05 h. Under above conditions, the iron content of Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) reached 16.03%. The IR and SEM indicated that Rehmannia glutinosa Libosch polysaccharide was combined with iron (Ⅲ). Rehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) could be dissolved and reduced easily as shown by in vitro dissolution experiment and reducing experiment, and the cumulative release percentage of iron (Ⅲ) was more than 80% within 60 min in stimulated gastrointestinal liquid. CONCLUSIONRehmannia glutinosa Libosch polysaccharide-iron (Ⅲ) has the advantages of high iron content and good solubility. It can be developed as a new iron supplementary.
CUI J, LI Y, YU P, et al. A novel low molecular weight Enteromorpha polysaccharide-iron(Ⅲ) complex and its effect on rats with iron deficiency anemia(IDA). Int J Biol Macromol, 2018, 108: 412-418.
XU L, MENG Y B, LIU Y, et al. A novel iron supplements preparation from Grifola frondosa polysaccharide and assessment of antioxidant, lymphocyte proliferation and complement fixing activities. Int J Biol Macromol, 2018, 108: 1148-1157.
GUO C J, WANG H, CHEN J J, et al. Research progress of polysaccharide iron complex in the treatment of renal anemia. Pharm Res(药学研究), 2019, 38(3):172-174,182.
JING Y S, ZHANG R J, WU L F, et al. Research progress in the structural characteristics and biological activity of polysaccharide-iron complex. Food Res Dev(食品研究与开发), 2019, 40(22):203-208.
NIE S P, TANG W, YIN J Y, et al. Research progress on structure and functional activities of food-derived polysaccharides. J Chin Inst Food Sci Tech(中国食品学报), 2018, 18(12):1-12.
XU L L, ZHAN X R, ZENG Z W, et al. Study on preparation and chemical characterization of iron(Ⅲ)- Polygonatum odoratum polysaccharides complex. Chin Pharm J(中国药学杂志), 2012, 47(5):331-334.
CUI Y, FANG X N, WANG H X, et al. Study on the blood enrichment of three different processed products of radix Rehmanniae. Lishizhen Med Mater Med Res(时珍国医国药), 2009, 20(1):20-22.
MIAO M S, WANG Z M, SUN Y H. Effect of Rehmannia glutinosa polysaccharide on blood picture and cytokine level in rats with blood deficiency. Chin Med Pharmacol Clin(中药药理与临床), 2007, 23(1): 39-40.
LIU P J, MIAO M S, GAO J L. Effect of gross polysaccharide of radix Rehmanniae preparata on levels of whole blood cells and serum macrophage colony stimulating factor in mice with both qi and blood deficiency. Chin J Tiss Eng Res(中国组织工程研究与临床康复), 2008, 12(38): 7543-7546.
LIU F J, CHENG J P, ZHAO X N, et al. Effects of Rehmannia glutinosa polysaccharide on hematopoietic stem cells, progenitor cells and peripheral blood picture of normal rats. Chin Med Pharmacol Clin(中药药理与临床), 1996, 12(2): 12-14.
HUANG X, LIU J, LIU H X. Influence of Rehmannia glutinosa polyses on model mice of deficiency of blood. China J Chin Mater Med(中国中药杂志), 2004, 29(12): 1168-1170.
ZHENG X K, HOU W W, DUAN P F, et al. Immunomodulatory effect of prepared radix Rehmanniae extract in vitro. Chin Pharm J(中国药学杂志), 2012, 47(24):1995-2000.
PEI X H, LI Y X, LI J. Study on synthesis and physico-chemical character of radix Rehmaannia polysaccharide-Fe(Ⅲ)complex. Henan Sci(河南科学), 2005, 23(4):499-501.
ZHAN X L, TIAN H W, WANG J. Determination of iron content in iron polysaccharide complex by uv-vis. Pharm Res(药学研究), 2015, 34(11):645-647.
JING Y S, ZAHNG R J, WU L F, et al. Preparation, structural characteristics and bioactivity of polysaccharide-iron complex from Radix glehniae. Food Sci(食品科学), 2017, 39(24):65-70.
LIU T. Studies on the Preparation and Characterization of Auricularia auricula polysaccharide-iron complex and its Anti-iron deficiency anemia. Changchun:Jilin Agricultural University, 2019.
TANG D L, SUN W Q, ZENG H T. Studies on the preparation, properties of alginate-iron(Ⅲ) complex. Chin Pharm J(中国药学杂志), 2016,51(17):1513-1518.
Ch. P(2015) Vol Ⅳ(中国药典2015年版.四部). 2015: 406.
ZHAO P, ZHANG T T, SONG X, et al. Carboxymethylation process and antioxidant ability of polysaccharides from Acanthopanax obouatus Hoo. Chem Ind Eng Prog(化工进展), 2015, 34(1):219-223.
REN G M, XU X H, QU J J, et al. Process optimization for synthesis of Ganoderma lucidum polysaccharides-iron(Ⅲ) complex base on response surface methodology. J Chin Inst Food Sci Tech(中国食品学报), 2016, 16(2):98-105.
CHEN T T, LI S Y, QIN T T, et al. Formulation optimization of alginate mPEG-b-PLGA nanoparticles by Box-Behnken response surface method. Chin Pharm J(中国药学杂志), 2019, 54(19):1590-1598.
LU Q, XU L, MENG Y, et al. Preparation and characterization of a novel Astragalus membranaceus polysaccharide-iron (Ⅲ) complex. Int J Biol Macromol, 2016, 93: 208-216.