Relationship Between Antioxidant Activity and Spectrum-Effect of Hawthorn Leaf Extracts
LI Yan-rong1, ZHOU Wei-wei1,2, WANG Zi-yi1, ZHAO Sheng-nan1, PAN Hai-feng1*
1. Hebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical College, Chengde 067000, China; 2. Chengde City Food and Drug Inspection and Testing Center, Chengde 067000, China
Abstract：OBJECTIVE To select the optimal extract of Hawthorn leaves based on the antioxidant capacity of different extracts in vitro for the determination of total flavonoids content and the study of fingerprint, and establish the spectrum-effect relationship. METHODS With vitamin C as the positive control, 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2, 2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) clearance rates were used to test the antioxidant activities of different extracts of Hawthorn leaves, and the extract with the strongest antioxidant activity was selected and used as the research object. UPLC analysis for determination of the content of total flavonoids was performed on Waters ACQUITY UPLC CSHTM C18(2.1 mm×100 mm, 1.7 μm) column using 0.1% formic acid water and acetonitrile as mobile phase. Gadient elution was conducted at the flow rate of 0.15 mL·min-1, the injection volume was 3 μL, the column temperature was 30 ℃, and the detection wavelength was 320 nm. The UPLC fingerprint was established. The spectrum-effect relationship was studied by using bivariate correlation analysis and grey correlation method. RESULTS Four kinds of extracts had certain antioxidant activity, among which hawthorn leaf extract listed in the Chinese Pharmacopoeia 2015 edition had the strongest ability to scavenge DPPH and ABTS free radicals (r=-0.896, r=-0.808). A total of 33 peaks were identified by UPLC fingerprint. They were highly correlated with the scavenging ability for free radicals and the correlation coefficients were all greater than 0.62. CONCLUSION This study provided the basis for the quality control of Hawthorn leaf extract and its preparations.
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