Abstract：OBJECTIVE To study the preparation, structure, inclusion mechanism and antioxidant activity of γ-cyclodextrin (γ-CD) inclusion complex of baicalein. METHODS The inclusion complex of baicalein and γ-CD in solution was studied by ultraviolet-visible absorption spectroscopy, fluorescence spectroscopy and nuclear magnetic resonance (NMR). The solid inclusion complex of baicalein and γ-CD was prepared by coprecipitation method. The inclusion complex was characterized by infrared spectroscopy (IR). The inclusion constant (K) and inclusion ratio (n) of the inclusion complex were determined. RESULTS In solutions of different pH values, γ-CD had different inclusion ability to baicalein, and neutral medium was the most suitable for γ-CD to form inclusion complex with baicalein. In the range of experimental concentration, baicalein and γ-CD formed inclusion complex at ratio of 1∶1, and the inclusion ratio was 1 067. Baicalein/γ-CD inclusion complex had more stronger scavenging ability for free radical DPPH· than free baicalein. The structure of the inclusion complex was determined by NMR. CONCLUSION Under the experimental conditions, baicalein molecule is encapsulated into the cavity of γ-CD from the large mouth, forming a stable inclusion complex, and the antioxidant activity is increased.
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2020, Vol. 55 
