Abstract：OBJECTIVE To investigate the effects of arctigenin (ARG) on the proliferation, migration and invasion of gastric cancer cells and its possible mechanism. METHODS BGC-823 cells were cultured in vitro, and cells were treated with ARG at different concentrations (10, 20, 40 mg·L-1) for 24 h, transfected with si-SETDB1, and transfected with pcDNA-SETDB1 and 40 mg·L-1 ARG. The proliferation of gastric cancer BGC-823 cells was detected by MTT assay. The migration and invasion ability were detected by Transwell method. The proteins associated with proliferation, migration and invasion and SETDB1 protein were detected by Western blot. SETDB1 mRNA expression was detected by qRT-PCR. RESULTS ARG and transfected si-SETDB1 could inhibit the proliferation of gastric cancer BGC-823 cells, decrease the migration and invasion of BGC-823 cells, down-regulate the expression of cyclin D1, MMP-2, MMP-9 and MMP-14 protein,and up-regulate the expression of p21 and p27 protein.The expression of SETDB1 mRNA and protein were down-regulated. Overexpression of SETDB1 reversed the effects of ARG on proliferation, migration and invasion of gastric cancer BGC-823 cells and the related proteins cyclin D1, p21, MMP-2 and MMP-9. CONCLUSION ARG may inhibit the proliferation, migration and invasion of gastric cancer BGC-823 cells by regulating the expression of SETDB1.
杨振方, 王保卫, 刘大勇, 廖新伟, 陈小兵. 牛蒡子苷元通过调控SETDB1表达对胃癌细胞增殖、迁移和侵袭的影响及其机制研究[J]. 中国药学杂志, 2020, 55(19): 1596-1602.
YANG Zhen-fang, WANG Bao-wei, LIU Da-yong, LIAO Xin-wei, CHEN Xiao-bing. Arctigenin Influences Proliferation, Migration and Invasion of Gastric Cancer Cells by Regulating the Expression of SETDB1. Chinese Pharmaceutical Journal, 2020, 55(19): 1596-1602.
LIU C X, LIU W, JI Y C. Progress and prospect of quantum dot fluorescence laparoscopic surgery in the treatment of gastric cancer. Chin J Minim Inva Surg (中国微创外科杂志), 2018, 18(3):271-273.
ZHOU S F, SUN H X. Comparison of effects of mEOX and FOLFIRI regimen on the efficacy, serum tumor markers and prognosis of patients with advanced gastric cancer. Shandong Med(山东医药), 2018, 58(38):52-54.
LI Z, ZHANG K, MI D H, et al. Chemotherapy combined with thermotherapy for advanced gastric cancer: a systematic review. Chin J Cancer Prevent Treat(中华肿瘤防治杂志), 2018, 25(4):73-80.
DING D W, ZHANG Y H. Advances in anti-tumor Chinese medicine targeting the apoptotic pathway. Chin J Gerontol(中国老年学杂志), 2018, 38(1):239-241.
LEE J, IMM J Y, LEE S H. β-Catenin mediates anti-adipogenic and anticancer effects of arctigenin in preadipocytes and breast cancer cells. J Agric Food Chem, 2017, 65(12):2513-2520.
XU Y, LOU Z, LEE S H. Arctigenin represses TGF-β-induced epithelial mesenchymal transition in human lung cancer cells. Biochem Biophys Res Commun, 2017, 493(2):934-939.
WANG P, SOLORZANO W, DIAZ T, et al. Arctigenin inhibits prostate tumor cell growth in vitro and in vivo. Clin Nut Exp, 2017, 13(8):1-11.
CHEN B, WANG J, WANG J. A regulatory circuitry comprised of TP53, miR-29 family and SETDB1 in non-small cell lung cancer. Hepatology, 2018, 15(6):1-12.
FEI Q, SHANG K, ZHANG J, et al. Histone methyltransferase SETDB1 regulates liver cancer cell growth through methylation of p53. Nat Commun, 2015, 6(14):8651-8665.
ZHOU Y, CHEN Y. The effect of SETDB1 silence on growth of human renal carcinoma cells and its molecular mechanism. Chin Clin Res(中国临床研究), 2016, 29(7):896-899.
ZHANG H P, WANG J M. Effect of arctigenin on metastasis ability of rectal cancer cells. Fujian J Tradit Chin Med(福建中医药), 2012, 43(2):35-37.
QIU H, XIANG D. The advancement of microRNA regulating epithelial-mesenchymal transition in cholangiocarcinoma. J Med Postgra (医学研究生学报), 2017, 30(6):665-668.
SUN Y, TAN Y J, LU Z Z, et al. Arctigenin inhibits liver cancer tumorigenesis by inhibiting gankyrin expression via C/EBPα and PPARα. Front Pharmacol, 2018, 9(12):268-281.
XIAOBO Z, PAKIN S, SHIQIANG Z, et al. 3,3′-Diindolylmethane downregulates cyclin D1 through triggering endoplasmic reticulum stress in colorectal cancer cells. Oncol Rep, 2017, 38(1):569-574.
XU L, LIU S, WU X W, et al. Promoting effect of cyclin D1 overexpression on proliferation and epithelial mesenchymal transition of cervical squamous cell carcinoma SiHa cells. Chin J Pathol(中华病理学杂志), 2017, 46(3):187-192.
JUNG D B, KHURANA A, ROY D, et al. Quinacrine upregulates p21/p27 independent of p53 through autophagy-mediated downregulation of p62-Skp2 axis in ovarian cancer. Sci Rep, 2018, 8(1):2487-2499.
LU L, LI D S, BAI G.Effect of XB130 on human hepatocellular carcinoma HepG2 cells. China J Mod Med (中国现代医学杂志), 2017, 30(30):21-25.
YANG W, LIU S C, CHEN W H, et al. Effect of 1,25(OH)2 D3 on migratory,invasive ability and expression of MMP-2,9 and 14 of human pancreatic cancer cell line PANC-1. Shandong Med(山东医药), 2016, 56(34):17-19.
WANG X L, XU D Y, LIU G, et al. SETDB1 expression in breast cancer and the effect on the proliferation and migration ability of cancer cells. Acta Univ Med Anhui(安徽医科大学学报), 2016, 51(8):1081-1087.
CHEN K L, ZHANG F J, DING J, et al. Histone methyltransferase SETDB1 promotes the progression of colorectal cancer by inhibiting the expression of TP53. J Cancer, 2017, 8(16):3318-3330.