Abstract：OBJECTIVE To determine the total polysaccharides in the production processof fermented Cordyceps powder roduction, realize the content determination and quality control of theproduction process using near infrared pectroscopy. METHODS The phenol-sulfuric acid method was used to measure the total polysaccharides content in 30 batches various operating units of production process chain, such as strain culture, strain passage, strain fermentation, ferment filtration, ferment drying, ferment pulverizing, and ferment mixing,and combined with near infrared spectroscopy (NIRS) to establish a total polysaccharides partial least squares regression (PLSR) quantitative analysis model for the production process chain of fermented Cordyceps powder,and to analyze the quality control level of different operating units in the production process chain of Fermentation Cordyceps powder. RESULTS The total polysaccharides content of the esvarious Fermented Cordyceps powder in the production process chain is relatively stable. The root mean square errors of calibration and prediction (RMSEC, RMSEP) and the correlation coefficients of calibration and prediction (Rc, Rp) of the total polysaccharides PLSR model were 0.018 6%, 0.013 3%, 0.941 8 and 0.980 0, respectively. The absolute errorbetween the predicted value and phenol-sulfuric acid method measured value is small of the model, the fitting degree is good. CONCLUSION It can quickly analyze the quality of fermented Cordyceps powder and provide a reference for the quality control of total polysaccharides in the production process chain fermentation Cordyceps powder.
史畑女, 陈丽华, 管咏梅, 金晨, 朱卫丰. 发酵虫草菌粉生产过程多糖类近红外光谱预测模型与质量控制[J]. 中国药学杂志, 2020, 55(19): 1585-1589.
SHI Tian-nü, CHEN Li-hua, GUAN Yong-mei, JIN Chen, ZHU Wei-feng. Near-infrared Spectroscopy Prediction Model and Quality Control of Polysaccharides in the Production Process of Cordyceps Powder Fermentation. Chinese Pharmaceutical Journal, 2020, 55(19): 1585-1589.
WANG H, SHAN Y, SUN Z R. The utilization and analysis of present market situation for Cordyceps sinensis. Res Pract Chin Med(现代中药研究与实践), 2016, 30(6):83-86.
[2]
ZHANG H, LI Y, MI J, et al. GC-MS profiling of volatile components in different fermentation products of Cordyceps sinensis mycelia. Molecules, 2017, 22(10):1800.
[3]
YUE K, YE M, ZHOU Z, et al. The genus Cordyceps: a chemical and pharmacological review. J Pharm Pharmacol, 2013, 65(4):474-93.
[4]
OLATUNJI O J, TANG J, TOLA A, et al. The genus Cordyceps: an extensive review of its traditional uses, phytochemistry and pharmacology. Fitoterapia, 2018, 129:293-316.
[5]
TANG H, WEI W, WANG W, et al. Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality. Carbohydr Polym, 2017, 163:43-53.
[6]
XIANG F, LIN L, HU M, et al. Therapeutic efficacy of a polysaccharide isolated from Cordyceps sinensis on hypertensive rats. Int J Biol Macromol, 2016, 82:308-314.
[7]
LUO X, DUAN Y, YANG W, et al. Structural elucidation and immunostimulatory activity of polysaccharide isolated by subcritical water extraction from Cordyceps militaris. Carbohydr Polym, 2017, 157:794-802.
[8]
BI S, JING Y, ZHOU Q, et al. Structural elucidation and immunostimulatory activity of a new polysaccharide from Cordyceps militaris. Food Funct, 2018, 9(1):279-293.
[9]
ZHANG Q, OLATUNJI O J, CHEN H, et al. Evaluation of the anti-diabetic activity of polysaccharide from Cordyceps cicadae in experimental diabetic rats. Chem Biodivers, 2018, 15(8):e1800219.
[10]
WU D T, MENG L Z, WANG L Y, et al. Chain conformation and immunomodulatory activity of a hyperbranched polysaccharide from Cordyceps sinensis. Carbohydr Polym, 2014, 110:405-414.
[11]
JING Y, CUI X, CHEN Z, et al. Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris. Carbohydr Polym, 2014, 102:288-296.
[12]
DONG Y, HU S, LIU C, et al. Purification of polysaccharides from Cordyceps militaris and their anti-hypoxic effect. Mol Med Rep, 2015, 11(2):1312-1317.
[13]
ZHU Z Y, LIU X C, TANG Y L, et al. Effects of cultural medium on the formation and antitumor activity of polysaccharides by Cordyceps gunnii. J Biosci Bioeng, 2016, 122(4):494-498.
[14]
GUO L X, ZHONG W, JIANG L. Effects of Cordyceps militaris polysaccharide on antioxidant level and Bcl-2, Bax mRNA Expression in H22 tumor tissues of aging rats. J Guizhou Med Univ(贵州医科大学学报), 2019, 44(2):173-177.
[15]
CUI B B, LI J, LIU K C, et al. Anti-hepatic fibrosis mechanism of Cordyceps polysaccharide. Chin J Exp Tradit Med Form(中国实验方剂学杂志), 2019, 25(15):228-234.
[16]
GUO S D, CUI Y J, WANG R Z, et al. Study on separation and purification of Cordyceps militaris polysaccharides and cholesterol promoting reverse transport. Chin J Chin Mater Med(中国中药杂志), 2014, 39(17):3316-3320.
[17]
LIU B, YANG S, XIE H H, et al. Effects of fermented Cordyceps sinensis polysaccharide and liposome preparation on mouse S180 sarcoma. Lishizhen Med Mater Med Res(时珍国医国药), 2018, 29(12):2853-2855.
[18]
ZHU W F, ZHAO J Q, LIU X L, et al. Study on fingerprint of polysaccharides from Jinshuibao Capsule. Chin Tradit Herb Drugs(中草药), 2017, 48(16):3322-3326.
[19]
CHEN L H, ZHANG Q H, OU Y S, et al. Determination of polysaccharide content in Cordyceps fermentation broth. Lishizhen Med Mater Med Res(时珍国医国药), 2015, 26(12):2880-2882.
[20]
HAO J W, CHEN N D, CHEN C W, et al. Rapid quantification of polysaccharide and the main onosaccharides in Dendrobium huoshanense by near-infrared attenuated total reflectance spectroscopy. J Pharm Biomed Anal, 2018, 151:331-338.
[21]
NIE H Y, ZHANG Q H, YAN D M, et al. Optimization of extraction process for polysaccharide from fermented Cordyceps sinensis powder by response surface methodology. J Guangzhou Univ Tradit Chin Med(广州中医药大学学报), 2017, 34(2):250-253.
[22]
XUE J J, TANG Q J, LIU Y F, et al. Comparison and optimization of the methods for analyzing thewater soluble polysaccharides in Cordyceps militaris. Mycosystema(菌物学报), 2012, 31(3):443-449.
[23]
SHI Y, WANG G L, LIN R C. NIR determination of amino acid from Cordyceps sinensis. J Pharm Anal(药物分析杂志), 2007, 27(1):90-92.
[24]
YANG N L, CHENG Y Y, QU H B. Determination of mannitol in Cordyceps sinensis by artificial neural network-near infrared spectroscopy. Chin J Anal Chem(分析化学), 2003, 31(6):664-668.
[25]
WANG S P, LIU Q, LIU H M, et al. Rapid identification of Jinshuibao Capsules by NIR spectroscopy. Chin Pharm Aff(中国药事), 2013, 27(11):1140-1142.
[26]
GUO W L. Application of near infrared spectroscopy technology in fermentation processes . Changchun: Jilin University, 2010.
[27]
WANG H, TIAN H Y, ZHANG S L, et al. Portable medium-wave near-infrared spectrometer for nondestructive online detection of fresh pork cholesterol. Spectr Spectr Anal(光谱学与光谱分析), 2017, 37(6):1759-1764.
[28]
FU B H, WANG Y. Comparative study on chemical constituents of Cordyceps sinensis mycelia and natural Cordyceps sinensis. Chin Comm Doct(中国社区医师), 2018, 34(30):7,9.
[29]
CAO Y, LI Z G. Comparison of polysaccharide components and functional activities of fermentation and natural paecilomyces hepialid. Food Res Devel(食品研究与开发), 2017, 38(11):21-25.
[30]
ZHANG L, YUE H S, JU A C, et al. Construction of NIRS-based process analytical system for productionof salvianolic acid for injection and relative discussion. Chin J Chin Mater Med(中国中药杂志), 2016, 41(19):3569-3573.
[31]
LIANG N, ZHANG K L, LI S C, et al. Detection of moisture and total sugar content of red jujube based on diffuse reflection optical fiber spectroscopy. Jiangsu Agric Sci(江苏农业科学), 2018, 46(22):231-234.
[32]
XU N, WEI X, REN B, et al. Quantitative analysis of near-infrared spectroscopy and band selection of water adenosine in Fermented Cordyceps powder. Spectr Spectr Anal(光谱学与光谱分析), 2012, 32(7):1762-1765.
[33]
CHEN L H, WU Y, GUAN Y M, et al. Analysis of the high-performance liquid chromatography fingerprints and quantitative analysis of multicomponents by single marker of products of fermented Cordyceps sinensis. J Anal Methods Chem, 2018, 2018:5943914.
2020, Vol. 55 
