HPLC结合化学计量学方法用于不同生长年限甘草药材黄酮类成分特征图谱研究

doi:10.11669/cpj.2020.17.003

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摘要目的建立甘草药材黄酮类成分高效液相色谱(HPLC)特征图谱,结合化学计量学方法对不同生长年限甘草药材进行特征图谱分析,研究不同生长年限甘草药材之间的差异,为其质量控制提供科学的评价方法。方法采用Shiseido Capcell Pak C₁₈色谱柱(4.6 mm×250 mm,5 μm);流动相为乙腈(A)-0.1%甲酸水(B),梯度洗脱;流速1.0 mL·min^-1;检测波长280 nm;柱温:40 ℃。通过对照品比对,对建立的指纹图谱主要特征峰进行确认,采用Chem Pattern化学计量学软件对结果进行处理,运用相似度分析、主成分分析和聚类分析方法深入研究影响不同生长年限甘草黄酮类化学成分的关键指标。结果 4年生甘草药材质量最佳。甘草苷、芹糖甘草苷、甘草素、异甘草苷的差异显著,可作为区分不同生长年限甘草的关键性指标成分。结论通过化学计量学分析方法,建立的甘草药材黄酮类成分特征图谱可为甘草药材规范化栽培和标准提高提供参考。

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关键词 ：甘草, 黄酮, 生长年限, 特征图谱, 化学计量学

Abstract：OBJECTIVE To establish the HPLC specific chromatogram of licorice flavonoids based on chemometric analysis method, and search for differentiated components of Glycyrrhiza uralensis Fisch. cultivated for different years. METHODS The analysis was performed on a Shiseido Capcell Pak C₁₈ column(4.6 mm×250 mm,5 μm) by gradient elution with acetonitrile-0.1% formic acid solution as mobile phase at a flow rate of 1.0 mL·min^-1. The column temperature was maintained at 40 ℃ and the detection wavelength was set at 280 nm. The HPLC specific chromatograms of Glycyrrhiza uralensis Fisch. cultivated for different years were further evaluated by chemometric methods including similarity analysis (SA),hierarchical clustering analysis (HCA) and principal component analysis (PCA). RESULTS Four-years-old licorice was confirmed as the best one. Liquiritin, liquiritin apioside, liquiritigenin and isoliquiritin had significant differences, which can be used as key indicators to distinguish Glycyrrhiza uralensis Fisch. cultivated for different years. CONCLUSION This method can provide reference for standardized cultivation and quality standard improvement of licorice.

Key words： Glycyrrhiza uralensis Fisch. flavonoid growth years specific chromatogram chemometrics

收稿日期: 2019-12-20

PACS:

R284

基金资助:国家十二五“重大新药创制”专项资助(2014ZX09304307-002);国家新兴产业重大工程包(产业创新能力工程)中药标准化项目资助(ZYBZH-Y-ZY-45)

通讯作者: 马双成,男,博士,研究员,博士生导师研究方向:中药民族药质量控制与评价研究 Tel:(010)53852076 E-mail:masc@nifdc.org.cn; 魏锋,男,博士,研究员,硕士生导师研究方向:中药化学成分研究及质量评价控制研究 Tel:(010)53852020 E-mail:weifeng@nifdc.org.cn

作者简介: 陈佳,女,副研究员研究方向:中药质量控制研究

引用本文:

陈佳, 杨蕊, 张权, 王继永, 魏锋, 马双成. HPLC结合化学计量学方法用于不同生长年限甘草药材黄酮类成分特征图谱研究[J]. 中国药学杂志, 2020, 55(17): 1415-1420. CHEN Jia, YANG Rui, ZHANG Quan, WANG Ji-yong, WEI Feng, MA Shuang-cheng. Specific Chromatograms of Glycyrrhiza uralensis Fisch. Flavonoids in Different Growth Years by HPLC Coupled with Chemometric Analysis. Chinese Pharmaceutical Journal, 2020, 55(17): 1415-1420.

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http://journal11.magtechjournal.com/Jwk_zgyxzz/CN/10.11669/cpj.2020.17.003 或 http://journal11.magtechjournal.com/Jwk_zgyxzz/CN/Y2020/V55/I17/1415

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