Abstract：OBJECTIVE To establish an HPLC method for the determination of total(Ct) and free(Cf) concentrations of teicoplanin (TEIC) in plasma. METHODS For determing the free concentration, the plasma samples were prepared by ultrafiltration. A C18 column was used, with acetonitrile -0.01 mol·L-1 sodium dihydrogen phosphate (25∶75, pH adjusted to 3.3 by phosphoric acid) as the mobile phase, the detection wavelength was set at 240 nm, the column temperature was maintained at 35 ℃, and the flow rate was 1.0 mL·min-1. The specificity,linearity, the lower limit of quantitation, precession,recovery and stability of the developed method were validated. RESULTS The free concentration was linear with in 0.5-50 μg·mL-1, the lower limit of quantification was 0.5 μg·mL-1, and the method recovery rate was 94.63%-103.72%. The intra-day and inter-day precision(RSD) were all less than 4.00％; the linearity was good with in the total concentration of 1.562 5-100 μg·mL-1, the lower limit of quantification was 1.562 5 μg·mL-1, the method recovery rate was 94.55%-99.59%. The intra-day and inter-day precision(RSD) were all less than 4.00％. The TEIC was all stability at the conditions of maintaining at room temperature for 10 h,after freeze-thaw cycle and keeping at 4 ℃ for 72 h. CONCLUSION The method is simple, accurate, and sensitive, and is suitable for the clinical determination of total and free concentrations of teicoplanin and the study of pharmacokinetics.
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2020, Vol. 55 
