Optimization of Extraction Process of Thymol and Carvacrol in Origanum vulgare by Box-Behnken Design-Response Surface Methodology
ZENG Jian-wei1,2,4, ZHANG Zhen-li3, LI Xi-hai1,2, WU Zhang-qiang4*, CHU Jian-feng1,2, ZHANG Shu-juan5, TAN Chun-jiang1,2, MAO Jing-jie1,2, ZHU Xiao-qin1,2, LIN Ru-hui1,2,6
1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; 2. Fujian Provincial Key Laboratoty of Integrative Medicine on Geriatrics, Fuzhou 350122, China; 3. SIPO Patent Examination (Beijing) Center, Beijing 100160, China; 4. Fujian Zhongnongmu Bio-pharmaceutical Co. ,Ltd. , Longyan 364000, China; 5. Fujian Provincial Judicial Rehabilitation Hospital, Fuzhou 350007, China; 6. Qinghai Kangle Hospital, Xining 810000, China
Abstract：OBJECTIVE To optimize the extraction process of thymol and carvacrol in Origanum vulgare by Box-Behnken design-response surface methodology. METHODS On the basis of single factor experiment, taking the sum of extraction rates of thymol and carvicol as the evaluation index, Box-Behnken design was used to investigate the effects of ethanol concentration, liquid-solid ratio and medicinal powder on the extraction rate. RESULTS The optimal extraction parameters were as follows: ethanol concentration was 80%, liquid to solid ratio was 13∶1 (mL/g), medicinal powder passing through 40 meshes was used, and the highest extraction rate was 694.80 μg·g-1, with a small deviation from the predicted value. CONCLUSION The optimal extraction method is simple, with low cost and good predictivity, and it can provide experimental basis for further large-scale production of thymol and carvacrol in Origanum vulgare.
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2020, Vol. 55 
