Analysis of Fingerprint and Pattern Recognition of Different Parts of Panax notoginseng
MENG Zhe1,2,3, WANG Li-jun2,3, HUANG Yang2,3, JIANG Kun2,3, WANG Jue2,3, YIN Guo2,3, WANG Tie-jie1,2,3*
1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Shenzhen Institute for Drug Control, Shenzhen 518057, China; 3. Shenzhen Key Laboratory of Drug Quality Standard Research, Shenzhen 518057, China
Abstract：OBJECTIVE To establish a chemical pattern recognition method for Panax notoginseng (P. notoginseng) and classify the main root, rhizome, and rootlet. METHODS The fingerprints of P. notoginseng samples in three different parts were established based on HPLC method. The similarity was calculated by the Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine (2012 edition). The pattern recognition were carried out by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). RESULTS The HPLC fingerprint common pattern of 35 batches of P. notoginseng samples was established. Similarity values were in the range of 0.994 to 1.000, which indicated that similarity analysis could not classify them. The established PCA model could only identify the rhizome, besides PLS-DA can completely identify the three parts of P. notoginseng. Seven characteristic peaks, such as ginsenoside Rg1, ginsenosides Rb1 were screened as biomarkers. CONCLUSION The combination of HPLC fingerprint and chemical pattern recognition could provide a comprehensive reference for the quality control and quality evaluation of P. notoginseng.
孟哲, 王丽君, 黄洋, 江坤, 王珏, 殷果, 王铁杰. 中药三七不同药用部位的指纹图谱及模式识别研究[J]. 中国药学杂志, 2020, 55(7): 504-509.
MENG Zhe, WANG Li-jun, HUANG Yang, JIANG Kun, WANG Jue, YIN Guo, WANG Tie-jie. Analysis of Fingerprint and Pattern Recognition of Different Parts of Panax notoginseng. Chinese Pharmaceutical Journal, 2020, 55(7): 504-509.
LI S J. Compendium of Materia Medica (本草纲目) [M]. Beijing:Traditional Chinese Medicine Publishing House, 1999:626.
[3]
SHA M C, ZHOU Y F, ZHANG H Z, et al. Study on differences of chemical components in different parts of Panax notoginseng[J]. Mod Chin Med (中国现代中药), 2018, 20 (7):832-836.
[4]
WANG Y, SHEN L, JIANG K, et al. Quality assessment of the traditional Chinese medicine Amomum villosum by chemical pattern recognition technique[J]. Chin J Pharm Anal (药物分析杂志), 2016, 36(10):1863-1869.
[5]
CHEN S Y, FU Y, YU L N, et al. HPLC Fingerprint and chemical pattern recognition of Valeriana jatamansi[J]. Chin Pharm J(中国药学杂志), 2019,54(6):484-488.
[6]
YANG B Y, PENG L, YAN Y G, et al. Discriminant analysis of raw and honey baked Farfarae Flos from different regions by HPLC fingerprint analysis combined with chemical pattern recognition[J]. Chin Tradit Herb Drugs (中草药), 2018, 49 (11):4991-4997.
[7]
HAN S N, ZHANG X H, ZHOU P P, et al. The application of chemometrics in the study of the relationship between traditional Chinese medicine[J]. China J Chin Mater Med (中国中药杂志), 2014, 39(14):2595-2602.
[8]
WANG T J, LUO X, WANG W, et al. Chemical pattern recognition of traditional Chinese medicine gentian quality[J]. Acta Pharm Sin (药学学报), 1992, 27 (6):456-461.
[9]
YAO C, XIN H, LU T L, et al. Comparing quality of gardenia in different places by HPLC[J]. Chin Pharm J (中国药学杂志), 2017, 52 (1):63-67.
[10]
ZHANG C P, ZHANG Y X, LI C, et al. UPLC Fingerprints and multi-component chemical pattern recognition of Euodiae Fructus in different years [J]. Chin Tradit Herb Drugs (中草药), 2019, 50(11):2700-2707.
[11]
CHEN F F, QI H Y, SHI Y P. Fingerprint analysis of Codonopsis Radix by HPLC coupled with chemometrics analysis[J]. Chin Herb Med, 2013, 5(4):307-312.
[12]
SILVA A C, PONTES L F, PIMENTEL M F. Detection of adulteration in hydrated ethyl alcohol fuel using infrared spectroscopy and supervised pattern recognitionmethods[J]. Talanta,2012,93:129-134.
[13]
LIU Y, WANG Q, WANG F, et al. HPLC Fingerprint and chemical pattern recognization of Kudiezi Injection[J]. Chin Pharm J (中国药学杂志), 2013, 48(24):2097-2101.
2020, Vol. 55 
