Abstract：OBJECTIVE To investigate the absorption mechanism of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis and Siwu Decoction. METHODS The circulatory perfusion technique was used in this study, the concentrations of paeoniflorin and phenol red in intestinal absorption circulation were respectively determined by HPLC and UV. The effects of pH value, drug concentration, absorption site and P-gp on the absorption of paeoniflorin were investigated separately. RESULTS By comparing the absorption of paeoniflorin in Radix Paeoniae Alba, both of Radix Paeoniae Alba and Radix Angelica Sinensis, and Siwu Decoction, it was found that in the sample solution at the same absorption site, pH value and concentration (based on the concentration of paeoniflorin), the absorption, Ka and cumulative absorption of paeoniflorin in compound Siwu decoction group were significantly increased compared with the other groups (P<0.05), while t1/2 was significantly decreased (P<0.05). When combined with the inhibitor and inducer of P-gp, the absorption of paeoniflorin showed significant increase or decrease in the amount of absorption, Ka and cumulative absorption% compared with the control group (P<0.05), and t1/2 also showed significant decrease or increase (P<0.05). CONCLUSION The absorption of paeoniflorin could be affected by P-gp, and under the same conditions, the absorption of paeoniflorin in complicated Chinese herbal formula is better than that in the single herb and herb-pair.
张蒙蒙, 孙向明, 宋辉, 胡扬, 刘博男, 王昕蕾, 李文兰. 白芍、药对及复方中芍药苷在体肠吸收机制比较研究[J]. 中国药学杂志, 2020, 55(9): 715-721.
ZHANG Meng-meng, SUN Xiang-ming, SONG Hui, HU Yang, LIU Bo-nan, WANG Xin-lei, LI Wen-lan. Comparative Study in Situ on Rat Intestinal Absorption Mechanism of Paeoniflorin in Single Herb, Herb-pair, and Complicated Chinese Herbal Formula. Chinese Pharmaceutical Journal, 2020, 55(9): 715-721.
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2020, Vol. 55 
