Quality Control and Adulteration Detection of Cattle Bile Powder Based on Bile Acids
CAO Shuai1, XIA Jing1, FU Ze-xiao1, LI Jia-hao1, LI Li-min1, HU Wen-yan2, HU Qing1, MAO Xiu-hong1, JI Shen1*
1. Shanghai Institute for Food and Drug Control, Shanghai 201203, China; 2. China Biochemical Pharmaceutical Industry Association, Beijing 100054, China
Abstract：OBJECTIVE To establish a method for the quality control of cattle bile powder based on cholic acid components, evaluate the quality of commercially sold cattle bile powder, and analyze the possible adulteration. METHODS A method was established for simultaneous determination of cholic acid content in bile powder of cattle, sheep, pig, chicken, duck and other animals. Self-made samples and samples collected by enterprises were studied respectively. Based on self-made samples, the differences and content rules of bile powder of cattle, pig, sheep and other animals were investigated, and the quality and possible adulteration of samples collected by enterprises were evaluated. RESULTS An HPLC-ELSD method was established for simultaneous determination of 12 cholic acids in bile powder of cattle, sheep, pigs, chickens and ducks. By analyzing the determination results of 23 batches of self-made animal bile powder, the contents of cholic acid in cattle bile powder and other animal bile powder were determined. By analyzing the determination results of cattle bile powder samples collected from 23 enterprises, it was found that there were mainly two kinds of adulteration of cattle bile powder likely: sheep bile powder and cholic acid waste. Only 8 of the 23 batches of samples collected by enterprises met the requirements, and the qualified rate was only 34.8%. The quality of varieties needs to be improved and supervised urgently. CONCLUSION The method established in this study can effectively control the quality of cattle bile powder, identify possible adulteration of cattle bile powder, and provide reference and basis for improving the quality standard of cattle bile powder and strengthening market supervision.
曹帅, 夏晶, 付泽晓, 李家豪, 李丽敏, 胡文言, 胡青, 毛秀红, 季申. 基于胆酸类成分的牛胆粉质量控制与掺伪检测研究[J]. 中国药学杂志, 2020, 55(5): 383-388.
CAO Shuai, XIA Jing, FU Ze-xiao, LI Jia-hao, LI Li-min, HU Wen-yan, HU Qing, MAO Xiu-hong, JI Shen. Quality Control and Adulteration Detection of Cattle Bile Powder Based on Bile Acids. Chinese Pharmaceutical Journal, 2020, 55(5): 383-388.
HE J, LI J, ZHU S, et al. RP-HPLC Simultaneous determination of three tauro-conjugated cholic acids in Pulvis Fellis Suis [J]. Chin J Pharm Anal(药物分析杂志), 2012, 32(2):229-232.
[3]
TAN C M, LU J X, WANG Z N. Determination of taurohyodeoxycholic acid and glycohyodeoxycholic acid in Pulvis Fellis Suis by HPLC [J]. Chin Tradit Pat Med(中成药), 2016, 38(1):122-125
[4]
ZHANG Y H, LIU J Z, PENG X, et al. HPLC Comparison of bile acids in Fellis Ursi powder, Fellis Suis powder, Calculus Bovis powder, Fellis Caprinus powder and Fellis Galli powder [J]. Chin J Pharm Anal(药物分析杂志), 2009, 29(3):487-490.
[5]
ZHANG Y J, XIA J, CAO S, et al. Determination and comparison of multiple kinds of bi1e acids in three kinds of Calculus Bovis [J]. Tradit Chin Drug Res Clin Pharm(中药新药与临床药理), 2016, 27(4):546-551
[6]
SHI Y, GENG Y, ZHENG T J, et al. Determination of the major bile acids in pig bile powder by HPLC-ELSD and chemometric analysis of the whole chromatographic profiles [J]. Chin J Pharm Anal(药物分析杂志), 2017, 37(2):283-289.
[7]
SHI Y, ZHENG T J, WEI F, et al. Studies of pattern recognition of fingerprint profile of cattle bile powder and determination of multi-component in it [J]. China J Chin Mater Med (中国中药杂志), 2016, 41(13):2487-2492.
[8]
KONG W J, JIN C, XIAO X H, et al. Determination of multicomponent contents in Calculus Bovis by ultra-performance liquid chromatography-evaporative light scattering detection and itsapplication for quality control [J]. J Sep Sci, 2010, 33(10):1518-1527.
[9]
LI K, WANG W H, QI Y X, et al. Determination and comparison of content to six cholic acid derivatives in two kinds of Calculus Bovis by HPLC-ELSD assay [J]. Chin Pharm J(中国药学杂志), 2010, 45(8):626-629.
2020, Vol. 55 
