Abstract：OBJECTIVE To carry out HPTLC and HPLC fingerprint analysis of 18 batches of Ganoderma samples using two kinds of reference substance of Ganoderma extract, G. lucidum Extract Reference Substance(CZERS) and G. sinense Extract Reference Substance(ZZERS). METHODS HPTLC Fingerprint was used to analyze triterpene acids and sterols in Ganoderma with chloroform-acetonitrile-methanol-formic acid (13∶2∶0.5∶0.5, develop 3 times) and cyclohexane-ethyl acetate-methanol-formic acid (15∶5∶0.5∶0.5, develop 2 times) respectively. HPLC Fingerprint analysis was conducted using Kromasil 100-5 C18 column (4.6 mm×250 mm, 5 μm) kept at 25 ℃. Mobile phase A was acetonitrile and B was 0.02% phosphoric acid; gradient elution procedure was as follows: 0-40 min, 29%→33% A; 40-70 min, 33%→65%A; 70-105 min, 65%→100%A; 105-120 min, 100% A; flow rate was 1.0 mL·min-1. DAD detector was adopted with detection wavelength set at 244 nm. The injection volume was 10 μL. RESULTS By using ERS and fingerprint analysis, G. lucidum, G. sessile and G. lucidum could be distinguished. The components of G. lucidum in different species and growth patterns were different. CONCLUSION There are many varieties of G. lucidum, which can be divided into wild and artificial cultures, and the culture media of artificial culture are different, which leads to the difference of individual components of different G. lucidum. Fingerprint analysis based on ERS of specific varieties are more suitable for the overall quality control of G. lucidum.
JIANG Y F, MA J, CHEN Y G, et al. Research progress on active ingredients of Ganoderma sp [J]. Heilongjiang Agricul Sci (黑龙江农业科学), 2014(8):137-142.
YUAN D X, HOU J. Active component, pharmacologic action and application of Ganoderma [J]. Chin J Ethnom Ethnop(中国民族民间医药杂志), 2006, 79: 110-113.
LIN L, FANG N H, WU D. A survey on effective constituents of Ganoderma lucidum[J]. Chin Tradit Pat Med(中成药), 2002, 24(10):793-796.
NIU J F, FANG Z, WAGN G H, et al. The research advance on the effective constituents of Ganoderma spp.[J]. J Hebei Agricul Univ(河北农业大学学报), 2002, 25: 51-54.
LIU G Q, DING C Y, WANG X L. Mechanisms of anticancer action of triterpenoids from Ganoderma lucidum:a review of recent researches[J]. Mycosystema(菌物学报), 2007, 26(3):470-476.
KIMURA Y, TANIGUCHI M, BABA K. Antitumor and antimetastatic effects on liver oftriterpe--noid fractions of Ganoderma lucidum:mechanism of action and isolation of an active substance[J]. Antican Res, 2002, 22(6A):3309-3318.
DAI Z L, CHANG W. Research progress on bioactivity of Ganoderma polysaccharide [J]. J Anhui Tradit Chin Med Coll(安徽中医学院学报), 2005, 10(5):60-61.
QIAN X H. Determination of monosaccharides composition from Ganoderma lucidum polysaccharide by thinlayer chromatography [J]. Chin J Mod Appl Pharm(中国现代药物应用), 2013, 7(18):13-14.
DING P, ZHANG Y D, XU H H. Determination of ganoderic acid B in different parts of Ganoderma lucidum [J]. Chin Tradit Herb Drug(中草药), 2001, 32(4):310-312.
JIANG J G, NORIO NAKAMURA, BYUNG-SUN MIN, et al. Quantitative determination of bitter principles in specimens of Ganoderma lucidum using high-performance liquid chromatography and its application to the evaluation of Ganoderma Products [J]. Chem Pharm Bull, 2004, 52(6):688-695.
GU Z Y, WENG X C. RP-HPLC Fingerprint for quality assessment of Ganoderma lucidum [J]. J Shangh Univ(Natr Sci Ed)(上海大学学报:自然科学版), 2008, 14(6):652-655.
HUANG S Q, WEI G, YAO S, et al. Optimization and comparison of fingerprints of triterpenoids from Ganoderma lucidum and Ganoderma sinense [J]. J South Chin Univ Technol(Nat Sci Ed)(华南理工大学学报:自然科学版), 2010, 38(8):121-125.
YAN Y, NIE S P, CHEN Y, et al. Study on HPLC fingerprint of chloroform extract of Ganoderma lucidum [J]. J Food Sci Biotechnol(食品与生物技术学报), 2009, 28(5):589-597.
XING Z T, YU Q H, ZHANG J S, et al. Comparative study on triterpenes in different ganoderma species[J]. J Chin Med Mater(中药材), 2004, 27(8):575-576.
YAN Y Z, XIE P S, LAM W L, et al. Study on triterpenoic acids distribution in Ganoderma Mushrooms by automatic multiple development high performance thin layer chromatographic fingerprint analysis[J]. J AOAC Int, 2010, 93(5):1384-1389.
XIE P S,YAN Y Z. Chromatograhpic Fingerprints for the Detailed Illustration of the Quality of Herbal Medicines(中药色谱指纹图谱精细分析图集)[M]. Fuzhou: Fujian Science & technology publishing house, 2015:159-172.
CHEN P,JIN H Y,SUN L, et al. Application of extractive reference substance in holistic quality control of traditional Chinese medicine [J]. Chin J Pharm Anal(药物分析杂志),2016,36(2):185-195.