1. Agronomy College, Sichuan Agricultural University, Chengdu 611130, China; 2. College of Environment and Ecology, Chengdu University of Technology, Chengdu 610059, China; 3. Institute for New Rural Development, Sichuan Agricultural University, Ya′an 625014, China
Abstract:OBJECTIVE To identify morphological characteristics of Cyathula officinalis Kuan. and Cyathula capitata(Wall.) Moq.. METHODS Based on tissue morphology features, C. officinalis Kuan. and Cyathula capitata (Wall.) Moq. were differentiated by using tissue sectioning, optical microscope, scanning electron microscopy and transmission electron microscopy technology. RESULTSC. officinalis exhibited features, such as main roots, stems composed of four vascular bundles, relative rich trichomes, less green color, however, for C. capitata, hairy roots, stems composed of two vascular bundles and less trichomes were observed. Compared with low altitude condition, high-altitude condition may induce darker green color and formation of yellow spots and osmiophilic granules of leaves for C. capitata. CONCLUSION It provides a scientific approach to differentiate these two closely related medicinal plants.
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