Abstract：OBJECTIVE To establish a high performance liquid chromatography(HPLC) method for determining chromone components in Saposhnikoviae Radix, and compare the similarities and differences between bolting and non-bolting samples. METHODS Agilent Ecilpse Plus C18 column(4.6 mm×250 mm,5 μm) was used for chromatographic analysis, water and methanol were used as mobile phase for gradient elution at a flow rate of 1 mL·min-1. Detection was conducted at 254 nm. The column temperature was maitained at 35 ℃ and the injection volume was 10 μL. RESULTS There were 10 common peaks in the HPLC fingerprints of 8 batches of Saposhnikoviae Radix,with similarity above 0.94 in six batches of bolting and non-bolting samples and below 0.90 in two batches of second-stubble samples.The HPLC assay had good linearity for the four chromone components. The average recoveries for prim-O-glucosylcimifugin,cimicifugin, 5-O-methylvisamidol glycoside and sec-O-glu cosylhamaudol were 95.05%, 98.62%, 98.3% and 99.02%,and the RSDs of repeatability test were 1.60%, 1.77%, 1.24% and 3.09%, respectively. When comparing the bolting and non-bolting Saposhnikoviae Radix, the content of cimicifugin increased after bolting, the other three components and the total amount of the four chromone components did not have significant variation. CONCLUSION The establishment of HPLC fingerprint combined with simultaneous determination of four chromone components provides a more comprehensive reference for the quality control and quality evaluation of Saposhnikoviae Radix. There are no obvious differences in chromone components between bolting and non-bolting Saposhnikoviae Radix, but there is significant difference between second-stubble and first-stubble Saposhnikoviae Radix.
刘丽娜, 张南平, 金红宇, 马双成. 野生抽薹与未抽薹防风中色原酮类成分比较研究[J]. 中国药学杂志, 2020, 55(8): 637-642.
LIU Li-na, ZHANG Nan-ping, JIN Hong-yu, MA Shuang-cheng. Comparison of Chromone Components and Their Contents between Bolting and Non-bolting Saposhnikoviae Radix. Chinese Pharmaceutical Journal, 2020, 55(8): 637-642.