HPLC Fingerprints of Different Polarity Fractions of Achyranthes bidentata of Different Specifications
JI Liang1,2, LIU Qian-ru1, LIANG Xian-kui1,2, LEI Jing-wei1,2*, ZHANG Zhen-ling1, CHEN Sui-qing1, GONG Hai-yan1,2
1. Henan University of Chinese Medicine, zhengzhou 450046, China; 2. Henan Province Traditional Chinese Medicine Quality Control and Evaluation Engineering Technology Research Center, zhengzhou 450046, China
Abstract：OBJECTIVE To study and compare the HPLC fingerprints of different polar parts of Achyranthes bidentata of different specifications, investigate their internal quality differences, and provide reference for the standard improvement and clinical use of the medicinal material. METHODS The Achyranthes bidentata samples were refluxed with 75% ethanol, the extract was dissolved with 40 mL water and then,extracted in turn with petroleum ether, chloroform, ethyl acetate and n-butanol, each extracted phase and aqueous phase were obtained and concentrated. The HPLC fingerprint of each part was collected. The data was analyzed by similarity and comprehensive clustering. Meanwhile, the fingerprints of different parts were compared and analyzed. RESULTS Eleven common peaks were determined in the HPLC fingerprints of petroleum ether fraction and chloroform extract fraction, 10,19,and 8 common peaks were determined in the HPLC fingerprints of ethyl acetate fraction, n-butyl alcohol fraction,and water extract respectively. The similarity of the fingerprints of chloroform parts was quite low.The similarity of the fingerprints of other parts was higher,all above 0.9. The difference in the fingerprints of petroleum ether parts was mainly reflected in the peak height. The chemical compositions and peak heights of ethyl acetate, chloroform and water extracts were different. Comprehensive cluster analysis could distinguish different specifications of Achyranthes bidentata. CONCLUSION The internal qualities of different specifications of Achyranthes bidentata are different. HPLC fingerprint established in this experiment can comprehensively reflect the chemical components distribution of Achyranthes bidentata of different specifications. It provides reference for the whole quality evaluation of different specifications of Achyranthes bidentata.
纪亮, 刘倩茹, 梁献葵, 雷敬卫, 张振凌, 陈随清, 龚海燕. 不同规格怀牛膝不同极性部位HPLC指纹图谱[J]. 中国药学杂志, 2020, 55(8): 580-587.
JI Liang, LIU Qian-ru, LIANG Xian-kui, LEI Jing-wei, ZHANG Zhen-ling, CHEN Sui-qing, GONG Hai-yan. HPLC Fingerprints of Different Polarity Fractions of Achyranthes bidentata of Different Specifications. Chinese Pharmaceutical Journal, 2020, 55(8): 580-587.
HU T T, ZHANG Z L. Chemical composition, pharmacological action and storage of Achyranthes bidentata[J]. Chin J Gerontol(中国老年学杂志),2016,36(13):3321-3322.
CHAO Z M, SHANG E J, HE B, et al. Study on chemical constituents of Achyranthes bidentata [J]. Chin Pharm J(中国药学杂志),1999,34(9):587-588.
DONG Y, WANG Q, QI W Y, et al. Analysis on research progress in the product development and patent technology of Achyranthes bidentata in China [J]. Pharm Biotechnol(药物生物技术),2018,25(1):51-55.
TIAN S, MIAO M S. Research on chemistry, pharmacology and application characteristics of Achyranthes bidentata [J]. China J Chin Med(中医学报),2014,29(8):1186-1188.
ZHANG Z L, SHI Y B, CHEN H, et al. Comparison of oleanolic acid content before and after sunburn with different grades of Achyranthes bidentata wine [J]. J Chin Med Mater(中药材),2008,31(5):650-651.
State Administration of Traditional Chinese Medicine of the People's Republic of China. Specifications and standards for 76 kinds of medicinal materials(七十六种药材商品规格标准)[S]. 1984:3.
Ch.P(2015). Vol Ⅰ(中国药典 2015年版.一部)[S]. 2015:73.
LI J. Research progress of Achyranthes bidentata [J]. Guide China Med (中国医药指南),2013,11(10):462-463.
YAN L, YAN M L, WW L H, et al. Anti-inflammatory effect of split fractions of radix Achyranthes bidentata [J]. Inform Tradit Chin Med(中医药信息),2016,33(6):8-11.
LIU W, ZHANG W N, PEI J, et al. Grey relational analysis of the relationship between variety and quality of Radix Cyathulae[J]. Chin Pharm J(中国药学杂志),2014,49(20):1796-1801.
LI J, BI Z M, XIAO Y J, et al. Studies on triterpenoid saponins from Achyranthese bidentata Bl[J]. Chin Pharm J(中国药学杂志),2007,42(3):178-180.
LIN D Z, WANG G S, YANG X H, et al. Studies on steroid constituents of Achyranthes bientata Bl [J]. Chin Pharm J(中国药学杂志),2006,41(17):1295-1297.
WANG R, SHEN C, WU H, et al. Anti-inflammatory and analgesic effects of different extracts of Achyranthes bidentata Radix and their effects against delayed-type hypersensitivity[J]. J Anhui Univ Chin Med(安徽中医药大学学报),2016,35(3):71-75.
XU Y, CAO J, WANG Y M, et al. Simultaneous determination of 3 kinds of components in Gardenia by high-performance liquid chromatography under different UV-vis wave length [J]. Acta Pharm Sin(药学学报),2003,38(7):543-546.