Anti-breast Cancer Effect of Ononin and Its Mechanism in Vitro
ZUO Fang1, CHEN Cheng2, REN Qian3
1. Department of Surgical Oncology, The Second Hospital of Baoding, Baoding 071051, China; 2. Department of Galactophore, The First Hospital of Lanzhou University, Lanzhou 730000, China; 3. Department of Gastroenterology, The First Hospital of Lanzhou University, Gansu Provincial Key Laboratory of Gastrointestinal Diseases, Lanzhou 730000, China
Abstract：OBJECTIVE To investigate the anti-breast cancer effect of ononin and its mechanism in vitro. METHODS After treating with different concentration of ononin, the MCF-7 cells viability was determined by MTS, EdU and Hoechst were used to measure the proliferative ability and apoptotic rate of MCF-7, respectively. The invasion and migration of MCF-7 were determined by Transwell assays. The protein expression including PCNA, apoptosis-related proteins (Bax and Bcl-2) and MMP-9 were evaluated by Western-blotting. RESULTS Ononin inhibited the cells viability of MCF-7 in a dose-and time-dependent manner. The EdU positive cells were decreased, Hoechst positive cells were increased, invasive and migration cells were decreased after treating with ononin in MCF-7. Moreover, ononin up-regulated Bax expression and down-regulated Bcl-2, PCNA as well as MMP-9 expression. CONCLUSION Ononin could inhibit the MCF-7 cells proliferation, promote cells apoptosis, decrease cells invasion and migration, which may involve the regulative effect on PCNA, Bax /Bcl-2 and MMP-9 expression.
左方, 陈诚, 任茜. 芒柄花苷的体外抗乳腺癌作用及机制研究[J]. 中国药学杂志, 2020, 55(3): 194-198.
ZUO Fang, CHEN Cheng, REN Qian. Anti-breast Cancer Effect of Ononin and Its Mechanism in Vitro. Chinese Pharmaceutical Journal, 2020, 55(3): 194-198.
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2020, Vol. 55 
