Quality Evaluation of Chrysanthemum indicum Based on Fingerprint, Pattern Recognition and Simultaneous Determination of Multi-Component
ZHANG Li-xian1,2, LI Zi-hong1,2, SONG Meng-jiao1,2, WEI Yue1,2*, LI Fei-fei1,2, CAO Jing-ya1,2, LI Zhi-ning1,2, NING Er-juan1,2, CHEN Xin1,2
1. Department of Analysis Biological Technology Development Center of Henan Province, Zhengzhou 450002, China; 2.Henan Cogo Testing and International Standardizing Technology Co. Ltd., Zhengzhou 450002, China
Abstract：OBJECTIVE To establish a method based on HPLC fingerprint, pattern recognition and simultaneous determination of multi-component for quality evaluation of Chrysanthemum indicum, and investigate its feasibility and accuracy. METHODS HPLC fingerprints of 21 batches of Chrysanthemum indicum were established, similarity analysis, principal component analysis and cluster analysis were performed on fingerprints of Chrysanthemum indicum by TCMYS fingerprint software, and 12 common chromatographic peaks in fingerprints were identified, and 12 components were quantified by external standard method. RESULTS The HPLC fingerprints of 21 batches of Chrysanthemum indicum were similar, and their classification trend was found. Seventeen common peaks were found and 12 of them were identified as neochlorogenic acid, chlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, cynaroside, isochlorogenic acid C, isochlorogenic acid A, (1S, 3R, 4R, 5R)-3, 4-bis[[(E)-3-(3, 4-dihydroxyphenyl)prop-2-enoyl]oxy]-1, 5-dihydroxycyclohexane-1-carboxylic acid, linarin, luteolin, apigenin and acacetin. Good linear relationship (r≥0.999 6) was shown for the external standard method, the average recoveries were between 95.84% and 102.46%, and the RSDs were all below 2.72%. CONCLUSION Combination of HPLC fingerprint, pattern recognition and simultaneous determination of multi-component are accurate and reliable for quality evaluation of Chrysanthemum indicum, which can provide important information for the overall quality evaluation of Chrysanthemum indicum.
张丽先, 李自红, 宋梦娇, 魏悦, 李飞飞, 曹静亚, 李智宁, 宁二娟, 陈欣. 基于指纹图谱、模式识别及多成分同时定量的野菊花质量评价研究[J]. 中国药学杂志, 2020, 55(3): 183-188.
ZHANG Li-xian, LI Zi-hong, SONG Meng-jiao, WEI Yue, LI Fei-fei, CAO Jing-ya, LI Zhi-ning, NING Er-juan, CHEN Xin. Quality Evaluation of Chrysanthemum indicum Based on Fingerprint, Pattern Recognition and Simultaneous Determination of Multi-Component. Chinese Pharmaceutical Journal, 2020, 55(3): 183-188.
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2020, Vol. 55 
