Abstract：OBJECTIVE To investigate intermedin protects against at rat renal tubular epithelial cells(NRK-52E) hypoxia-reoxygenation injury through suppresses endoplasmic reticulum stress(ERS)-related apoptosis. METHODS The NRK-52E cells were divided into 4 groups. One of them was control group; the other three model groups were exposed to H/R condition, following the intervention of single H/R, primitive vector and highly expressed was used IMD vector, respectively. The cell survival rate was detected by MTT; annexin V-FITC/PI double-stained cell apoptosis detection kit for detection of apoptosis; real-time PCR and Western-blot were used to detect expression of ERS molecules such as GRP78, CHOP and Caspase 12. ER specific resident fluorochrome dapoxyl was used to observe ER morphological changes. RESULTS MTT RESULTS showed that the survival rate of NRK-52E cells in H/R group was significantly decreased compared with the control group, and the cell survival rate was increased significantly in IMD group. The apoptosis rate of H/R increased significantly, and the mRNA and protein expressions of GRP78, CHOP and Caspase 12 in H/R group were all up-regulated significantly, IMD gene transfer markedly decreased the apoptosis index compared with that of H/R. The mRNA and protein expressions of GRP78, CHOP and Caspase 12 in IMD group were all down-regulated significantly. Dapoxyl staining demonstrated that the structure of the ER was severely destroyed in H/R, the fluorescence density showed nonuniform distribution, accompanied by notable vacuoles in the ER; Over-express IMD decreased the injury of the ER. CONCLUSION During rat renal tubular epithelial cells NRK-52E H/R injury may occur activation of ERS apoptotic pathway, IMD inhibites ERS-related apoptosis, reduces renal tubular epithelial cell apoptosis and reduces renal IRI.
亢晶, 王娟娟, 田继华, 王桂琴, 王艳红. 中介素通过抑制内质网应激保护缺氧复氧损伤的大鼠肾小管上皮细胞[J]. 中国药学杂志, 2020, 55(2): 105-110.
KANG Jing, WANG Juan-juan, TIAN Ji-hua, WANG Gui-qin, WANG Yan-hong. Protective Effect of Intermedin on Rat Renal Tubular Epithelial Cells Hypoxia-reoxygenation Injury by Inhibiting Endoplasmic Reticulum Stress. Chinese Pharmaceutical Journal, 2020, 55(2): 105-110.
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2020, Vol. 55 
