Detection Method and Degradation Dynamics of Chlorfenapyr in Honeysuckle Under Field Conditions
WANG Peng-si1, XUE Jian1*, HOU Shao-yan1, JIN Hong-yu2, MA Shuang-cheng2
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China; 2. National Institute for Food and Drug Control, Beijing 100050, China
Abstract：OBJECTIVE To develop a gas chromatography (GC) method for determination of chlorfenapyr in flower buds of Lonicera japonica Thunb, and study the degradation dynamics of chlorfenapyr in honeysuckle collected through field experiment, thus providing theoretical references for the application of chlorfenapyr in practical production of honeysuckle. METHODS Honeysuckle samples were extracted with acetone using a vortex mixer and then cleaned up by passing through a neutral alumina chromatography column, followed by detection by gas chromatography with electronic capture detector (GC-ECD). External standard method was used for quantification. RESULTS At fortified levels of 0.02-0.5 mg·kg-1, the average recoveries of chlorfenapyr in honeysuckle were from 83.9% to 97.6%, with RSDs of 0.6%-9.2%. The limit of detection was estimated to be 7.3 μg·kg-1. The degradation dynamics of chlorfenapyr in honeysuckle conformed to first-order dynamic equation. The degradation half-life of chlorfenapyr was 2.3 d. CONCLUSION The method developed in this experiment is simple, rapid, reliable and in line with the requirements of pesticide residue analysis. The degradation rate of chlorfenapyr in honeysuckle is relatively rapid. Chlorfenapyr belongs to pesticides which are liable to degradation.
王鹏思, 薛健, 侯少岩, 金红宇, 马双成. 虫螨腈在中药材金银花上的残留检测方法与消解动态研究[J]. 中国药学杂志, 2020, 55(1): 58-61.
WANG Peng-si, XUE Jian, HOU Shao-yan, JIN Hong-yu, MA Shuang-cheng. Detection Method and Degradation Dynamics of Chlorfenapyr in Honeysuckle Under Field Conditions. Chinese Pharmaceutical Journal, 2020, 55(1): 58-61.
HE B, LIU Y, LI C H, et al. Quality control of Lonicerae Japonicae Flos by multi-marker components quantitative fingerprint . Chin Pharm J(中国药学杂志),2015,50(14):1237-1242.
[3]
HE H Y, ZHANG D, JIANG H. Determination of chlorogenic acid and galuteolin in different kinds of Lonicera japonica Cultivated in Hailuogou scenic area . Nat Prod Res Dev(天然产物研究与开发), 2017, 29(4):616-622.
[4]
SUN Y, XUE M, ZHANG X, et al. Population dynamics and control techniques of aphids on honeysuckle . China J Chin Mater Med(中国中药杂志), 2013, 38(21):3676-3680.
[5]
WU C F, MA X M. Present situation and countermeasures to control pests and diseases of Lonicera japonica in Henan . Hubei Agric Sci(湖北农业科学), 2012, 51(13):2732-2735.
[6]
CHAI J P, XIE D Y, CHEN S. The test on toxicity of a newly pesticide chlorfenapyr to silkworm . Bull Sericul(蚕桑通报), 2010, 41(1):16-18.
[7]
WU C, LIU Y P, XIONG X M, et al. Study on residues and dissipation of chlorfenapyr in Chinese cabbage and soil . Hunan Agric Sci(湖南农业科学), 2016, (4):72-75.
[8]
LI R J, DING R Y, LI G D, et al. Determination of degrading dynamics of chlorfenapyr residue in Brassica chinensis L. by GC . Shandong Agric Sci(山东农业科学), 2012, 44(7):104-107.
[9]
ZENG M S, LIU F J, HUANG H S, et al. Security analysis of chlorfenapyr residues in tea plantation . Agrochemicals(农药), 2014, 53(6):432-435.
[10]
LIU Z J, GU S B, WANG K Y, et al. Determination of residual toxicity and degradation dynamics of chlorfenapyr in mulberry leaves . Acta Sericologica Sin(蚕业科学), 2008, 34(4):597-602.
[11]
MAI Y L, LIU H M, LIU C L, et al. Study on residue dynamics of chlorfenapyr in cucumber and amaranth . J South China Agricul Univ(华南农业大学学报), 2007, 28(1):67-69.
[12]
The ministry of agriculture of the People′s Republic of China. NY/T788-2004《农药残留试验准则》[S]. 2004.
[13]
LIU F, GUO Z Y, LIU H L, et al. Degradation dynamics of chlorfenapyr in paddy-field . Hunan Agric Sci(湖南农业科学), 2012, (7):71-73.
[14]
WANG Y J, XUE J, JIN H Y, et al. Residual regularity and dietary risk assessment of sulfoxaflor in honeysuckle . Chin Tradit Patent Med(中成药), 2017, 39(7):1534-1536.
[15]
WANG Y J, XUE J, GU Y, et al. Research on sulfoxaflor detection method and its degradation dynamics in Loincerae Japonicae Flos . J Chin Med Mater(中药材), 2016, 39(11):2441-2444.
[16]
HUANG-FU W G, CHEN G, YANG T. Residue detection of chlorfenapyr in vegetables . Acta Agricul Zhejiange(浙江农业学报), 2007, 19(2):119-122.
2020, Vol. 55 
