Determination of Lamotrigine, Olanzapine and Quetiapine in Human Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring
YANG Xi, LI Lu, HE Ling-juan, HE Li-na, LOU Yan, LU Xiao-yang*
Pharmacy Department of the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China
Abstract：OBJECTIVE To establish a highly sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneous determination of the concentrations of lamotrigine, olanzapine and quetiapine in human plasma to provide guidance for clinical drug use. METHODS The plasma samples were precipitated by methanol, voriconazole was used as internal standard, and then gradiently eluted by ACQUITY UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) using mobile phase consisting of 0.1% formic acid solution and methanol. Multiple reaction monitoring (MRM) was conducted in ion detection mode using positive electrospray ionization source. RESULTS The linear ranges of the calibration curves for lamotrigine, olanzapine and quetiapine in human plasma were 0.5-20 μg·mL-1, 2-200 and 10-1 000 ng·mL-1, respectively. The method had good matrix effect, extraction recovery, accuracy, precision and stability, and was successfully applied to analyze plasma samples of 45 patients. CONCLUSION The UPLC-MS/MS method for determination of lamotrigine, olanzapine and quetiapine is proven to be a sensitive and reliable protocol for clinical therapeutic drug monitoring.
杨希, 李璐, 何玲娟, 何丽娜, 楼燕, 卢晓阳. 人血浆中拉莫三嗪、奥氮平、喹硫平浓度的UPLC-MS/MS分析方法建立及治疗药物监测[J]. 中国药学杂志, 2020, 55(1): 44-51.
YANG Xi, LI Lu, HE Ling-juan, HE Li-na, LOU Yan, LU Xiao-yang. Determination of Lamotrigine, Olanzapine and Quetiapine in Human Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring. Chinese Pharmaceutical Journal, 2020, 55(1): 44-51.
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